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Complement Component C5aR1 Antibody (347214) [Unconjugated]

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Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa­minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2‑10 µg/mL in the presence of cytochalasin‑B.
Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa­minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
347214
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3648U-100UG
Alexa Fluor 405
FAB3648V-100UG
Alexa Fluor 488
FAB3648G-100UG
Alexa Fluor 532
FAB3648X
Alexa Fluor 594
FAB3648T-100UG
Alexa Fluor 647
FAB3648R-100UG
Alexa Fluor 700
FAB3648N-100UG
Alexa Fluor 750
FAB3648S-100UG
Allophycocyanin
FAB3648A
Allophycocyanin/Cy7
FAB3648APCCY7
Biotin
FAB3648B
CoraFluor 1
FAB3648CL1
DyLight 350
FAB3648D
DyLight 405
FAB3648E
DyLight 488
FAB3648K
DyLight 550
FAB3648L
DyLight 594
FAB3648M
DyLight 650
FAB3648W
DyLight 680
FAB3648Y
DyLight 755
FAB3648Z
Fluorescein
FAB3648F
HRP
FAB3648H
Janelia Fluor 525
FAB3648JF525
Janelia Fluor 549
FAB3648I
Janelia Fluor 585
FAB3648JF585
Janelia Fluor 635
FAB3648JF635
Janelia Fluor 646
FAB3648J
Janelia Fluor 669
FAB3648JF669
PE/Atto594
FAB3648PEATT594
PE/Cy5.5
FAB3648PECY55
PE/Cy7
FAB3648PECY7
PerCP
FAB3648C
Phycoerythrin
FAB3648P
mFluor Violet 450 SE
FAB3648MFV450
mFluor Violet 500 SE
FAB3648MFV500
mFluor Violet 610 SE
FAB3648MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB3648F
Carrier Free
FAB3648P
Carrier Free
FAB3648A
Carrier Free
MAB3648-SP
Carrier Free
FAB3648V-100UG
Carrier Free
FAB3648U-100UG
Carrier Free
FAB3648T-100UG
Carrier Free
FAB3648S-100UG
Carrier Free
FAB3648R-100UG
Carrier Free
FAB3648N-100UG
Carrier Free
FAB3648G-100UG
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Complement Component C5aR1 Antibody (347214) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
Met1-Val350
Accession # P21730
Specificity
Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
C5AR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 2-10 ug/mL
Publications
Read Publications using
MAB3648 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5aR1 Antibody (347214) [Unconjugated]

  • C5a anaphylatoxin chemotactic receptor
  • C5A
  • C5aR
  • C5a-R
  • C5AR1
  • C5ARC5a anaphylatoxin receptor
  • C5R1C5a ligand
  • CD88 antigen
  • CD88
  • complement component 5 receptor 1 (C5a ligand)
  • complement component 5 receptor 1
  • complement component 5a receptor 1
  • Complement Component C5a R1
  • Complement Component C5aR1

Background

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement Component C5aR1 Antibody (MAB3648)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, IHC-P.


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(1)
IHC-P
(1)
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(2)
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Bioinformatics

Gene Symbol C5AR1
Uniprot