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Complement C3c Antibody (4C6A8) - BSA Free

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Western Blot: Complement C3 Antibody (4C6A8) [NBP2-61855] - Analysis using C3C mAb against human C3C (AA: 1521-1649) recombinant protein. (Expected MW is 40.7 kDa)
Immunocytochemistry/ Immunofluorescence: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of Hela cells using C3C mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry-Paraffin: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of paraffin-embedded bladder cancer tissues using C3C mouse mAb with DAB staining.
Flow Cytometry: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of Hela cells using C3C mouse mAb (green) and negative control (red).
Western Blot: Complement C3 Antibody (4C6A8) [NBP2-61855] - Analysis using C3C mAb against HEK293 (1) and C3C (AA: 1521-1649)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Complement C3c Antibody (4C6A8) [NBP2-61855] - Analysis of paraffin-embedded ovarian cancer tissues using C3C mouse mAb with DAB staining.
ELISA: Complement C3c Antibody (4C6A8) [NBP2-61855] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
4C6A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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Complement C3c Antibody (4C6A8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Complement C3c (AA: 1521-1649) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
187.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Complement C3c Antibody (4C6A8) - BSA Free

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Complement C3c Products

Array NBP2-61855

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Bioinformatics

Gene Symbol C3