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Canine Complement C3 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Plasma, Serum
Standard Curve Range
6.25 to 200 ng/mL
Sensitivity
2.039

Order Details

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Canine Complement C3 ELISA Kit (Colorimetric) Summary

Description
C3 (Dog) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of C3 in dog serum or plasma.
Standard Curve Range
6.25 to 200 ng/mL
Sensitivity
2.039
Assay Type
Two-site enzyme-linked immunoassay
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C3

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Anti-Dog C3 ELISA Micro Plate: Twelve removable eight (8) well strips in well holderframe. Each well is coated with affinity purified anti-Dog C3.
  2. Chromogen-Substrate Solution: One vial containing 3,3',5,5'-tetramethylbenzidine(TMB) and hydrogen peroxide in citric acid buffer at pH 3.3
  3. Diluent Concentrate (Running buffer): One bottle containing 5X concentrated diluentrunning buffer.
  4. Dog C3 Calibrator: One vial containing a lyophilized Dog C3 calibrator.
  5. Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti- Dog C3antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  6. Stop Solution: One vial containing 0.3 M sulfuric acid.WARNING: Avoid contact with skin.
  7. Wash Solution Concentrate: One bottle containing 20X concentrated wash solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Canine Complement C3 ELISA Kit (Colorimetric)

  • Acylation Stimulating Protein
  • acylation-stimulating protein cleavage product
  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • C3
  • C3a anaphylatoxin
  • C3a
  • C3adesArg
  • C3b
  • C3bc
  • C3-beta-c
  • Complement C3 alpha chain
  • Complement C3 beta chain
  • complement C3
  • Complement C3b alpha' chain
  • Complement C3c alpha' chain fragment 1
  • Complement C3c alpha' chain fragment 2
  • Complement C3d fragment
  • Complement C3dg fragment
  • Complement C3f fragment
  • Complement C3g fragment
  • complement component 3
  • complement component C3
  • complement component C3a
  • complement component C3b
  • CPAMD1
  • EC 3.4.21.43
  • epididymis secretory sperm binding protein Li 62p
  • HEL-S-62p
  • prepro-C3

Background

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1-3). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1,4). The complement cascade consists of three main pathways: the classical, the lectin, and the alternative, all of which converge into a common pathway involving C3 cleavage by C3-convertases (1-6). Human Complement C3 is synthesized as a protein of 1663 amino acids (aa) in length with a theoretical molecular weight of ~185 kDa (5). Complement C3 is the most prevalent human complement protein in the serum, with a concentration of 1.2 mg/mL, and is predominantly produced by hepatocytes in the liver, but is also synthesized by blood cells and epithelial cells (3,5). Furthermore, the structure of C3 is comprised of an alpha-chain (110 kDa) and a beta-chain (75 kDa) linked by a disulfide bond (5). Cleavage of inactive C3 by C3-convertases produces active C3a, which functions as a mediator of inflammation, and C3b, which is an opsonin (1-4). In addition to amplification of complement response, C3 fragments serve multiple additional functions including chemotaxis, phagocytosis, adhesion, and immune modulation (3). Complement C3 serves dual purposes where it is involved in pathogenesis and immunity but, conversely, cellular damage results from unregulated C3 activation (5).

Both elevated levels and reduced levels of Complement C3 has been implicated in diseases pathologies (6). Deficiency in Complement proteins can result in autoimmune disorders including systemic lupus erythematosus, which is more often associated with C1 or C4 deficiency and only rarely with C3 deficiency (6). However, C3 deficiency typically results in increased risk of recurrent bacterial infections and glomerulonephritis, characterized by inflammation of the filtering glomeruli in the kidneys (6). Additionally, elevated levels of C3a and C4a is seen in patients with antiphospholipid antibody syndrome (6). Serum levels of C3 are also higher in rheumatoid arthritis cases (6). The complement system has become a target for drugs and therapeutics aimed at modulating innate immunity (7). For instance, compstatin is a peptide that binds to C3, inhibiting convertase activity and cleavage and can be used to treat diseases associated with uncontrolled C3 activation (7). C3-inhibitors and other complement inhibitors are a promising drug candidate for treatment of many diseases (7).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Merle, N. S., Church, S. E., Fremeaux-Bacchi, V., & Roumenina, L. T. (2015). Complement System Part I - Molecular Mechanisms of Activation and Regulation. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2015.00262

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Merle, N. S., Noe, R., Halbwachs-Mecarelli, L., Fremeaux-Bacchi, V., & Roumenina, L. T. (2015). Complement System Part II: Role in Immunity. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2015.00257

5. Sahu, A., & Lambris, J. D. (2001). Structure and biology of complement protein C3, a connecting link between innate and acquired immunity. Immunological Reviews. https://doi.org/10.1034/j.1600-065x.2001.1800103.x

6. Vignesh, P., Rawat, A., Sharma, M., & Singh, S. (2017). Complement in autoimmune diseases. Clinica Chimica Acta; International Journal of Clinical Chemistry. https://doi.org/10.1016/j.cca.2016.12.017

7. Mastellos, D. C., Yancopoulou, D., Kokkinos, P., Huber-Lang, M., Hajishengallis, G., Biglarnia, A. R., Lupu, F., Nilsson, B., Risitano, A. M., Ricklin, D., & Lambris, J. D. (2015). Compstatin: a C3-targeted complement inhibitor reaching its prime for bedside intervention. European Journal of Clinical Investigation. https://doi.org/10.1111/eci.12419

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Complement C3 ELISA Kit (KA1923). (Showing 1 - 1 of 1 FAQ).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

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Complement C3 - The Most Important Protein in the Complement System
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Bioinformatics

Gene Symbol C3