Complement C3 Antibodies
Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system that functions in immunity and clearance of pathogens. Human Complement C3 is synthesized as a protein of 1663 amino acids (aa) in length with a theoretical molecular weight of ~185 kDa. We offer several highly reviewed antibodies to detect Complement C3 that have been cited in 12+ publications. Browse our catalog of recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and Complement C3-conjugated antibodies. Our Complement C3 antibodies have been validated for multiple research applications including Affinity Purification, CyTOF-ready, ELISA, Flow Cytometry, Immunoassay, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Simple Western, and Western Blot. These Complement C3 antibodies are suitable for use in Human and Mouse. Each Complement C3 antibody is fully covered by Novus’ Guarantee+. Choose the anti-Complement C3 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for Complement C3 Antibodies
anti-Complement C3 antibody, anti-C3 antibody, anti-AHUS5 antibody, anti-ARMD9 antibody, anti-ASP antibody, anti-C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody, anti-complement component 3 antibody, anti-complement component C3 antibody, anti-CPAMD1acylation-stimulating protein cleavage product antibody
18 Results for "complement-c3" in Primary Antibodies
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Intracellular-NB200-540-img0005.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Flow-Cytometry-NB200-540-img0006.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-11H9-Immunohistochemistry-Paraffin-NB200-540-img0003.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0023.jpg "Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32080-img0019.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Mouse brain. C3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-32080-img0015.jpg)
![Western Blot: Complement C3 Antibody [NBP1-32080] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Western-Blot-NBP1-32080-img0021.jpg)
![Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows a specific band for Complement C3 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Simple-Western-NBP1-32080-img0009.jpg)
![Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunoprecipitation-NBP1-32080-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Immunocytochemistry-Immunofluorescence-NBP2-66994-img0002.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.")
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Analysis of HepG2 cells with C3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Flow-Cytometry-NBP2-66994-img0001.jpg)
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4 c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-flow-cytometry-1542024105623..jpg)
![Western Blot: Complement C3 Antibody (JF10-30) [NBP2-66994] -Western blot analysis of C3 on different lysates with Rabbit anti-C3 antibody at 1/1,000 dilution.Lane 1: Human liver tissue lysateLane 2: HepG2 cell lysateLane 3: HeLa cell lysateLane 4: Mouse liver tissue lysateLane 5: Mouse spleen tissue lysateLane 6: Rat liver tissue lysateLane 7: Rat spleen tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 187 kDaObserved band size: 108/37 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-western-blot-1542024105739..jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] -Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4 c. Goat Anti-Mouse IgG H&L (iFluor™ ) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-immunocytochemistry-immunofluorescence-15420241102..jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85492 (A) shows similar protein distribution across tissues to independent antibody NBP1-85493 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human adipose tissue shows moderate extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85492] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85492-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue, kidney, lung and testis using Anti-Complement C3 antibody NBP1-85493 (A) shows similar protein distribution across tissues to independent antibody NBP1-85492 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0009.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human adipose tissue shows strong extracellular space positivity in adipocytes.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human kidney shows strong extracellular space positivity in cells in tubules.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0007.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-85493] - Staining of human lung shows strong extracellular space positivity in macrophages.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-Immunohistochemistry-Paraffin-NBP1-85493-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249422664.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 stained by C3 antibody [HL1643] (NBP3-25389) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249435517.jpg)
![Western Blot: Complement C3 Antibody (HL1643) - Azide and BSA Free [NBP3-25389] - Rat plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (NBP3-25389) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25389_rabbit-complement-c3-mab-hl1643-azide-and-bsa-free-2120249362747.jpg)
![Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-262202415475630.jpg "Immunohistochemistry: Complement C3 Antibody (4D12) [NBP3-26471] - Image of Complement C3 Antibody (4D12) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Complement C3 Antibody (4D12) [NBP3-26471] - Positive Western Blot detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: Complement C3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 188 kDa Observed band size: 115 kDa](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-28220241140917.jpg)
![Flow Cytometry: Complement C3 Antibody (4D12) [NBP3-26471] - Overlay histogram showing HepG2 cells stained with Complement C3 Antibody (4D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26471_rabbit-complement-c3-mab-4d12-282202414183687.jpg)
![Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Western-Blot-H00000718-M01-img0007.jpg "Western Blot: Complement C3 Antibody (5F9) [H00000718-M01] - C3 monoclonal antibody (M01), clone 5F9 Analysis of C3 expression in A-431.")
![Immunohistochemistry-Paraffin: Complement C3 Antibody (5F9) [H00000718-M01] - Analysis of monoclonal antibody to C3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration at 3 ug/mL.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-Immunohistochemistry-Paraffin-H00000718-M01-img0005.jpg)
![ELISA: Complement C3 Antibody (5F9) [H00000718-M01] - Detection limit for recombinant GST tagged C3 is approximately 1 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-5F9-ELISA-H00000718-M01-img0008.jpg)
![Western Blot: Complement C3 Antibody (S06-2C2) [NBP3-19586] - Western blot detection of Complement C3 in human serum lysates using NBP3-19586. Predicted band size: 187kDa. Observed band size: 187, 115, 40, 34kDa.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-S06-2C2-Western-Blot-NBP3-19586-img0001.jpg "Western Blot: Complement C3 Antibody (S06-2C2) [NBP3-19586] - Western blot detection of Complement C3 in human serum lysates using NBP3-19586. Predicted band size: 187kDa. Observed band size: 187, 115, 40, 34kDa.")
![Immunohistochemistry: Complement C3 Antibody (8C3) [NBP3-26472] - Image of Complement C3 Antibody (8C3) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26472_rabbit-complement-c3-mab-8c3-26220241510615.jpg "Immunohistochemistry: Complement C3 Antibody (8C3) [NBP3-26472] - Image of Complement C3 Antibody (8C3) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Complement C3 Antibody (X1) [H00000718-M11-100ug] - Detection against Immunogen (37.95 kDa).](http://images.novusbio.com/fullsize/Complement-C3-Antibody-X1-Western-Blot-H00000718-M11-100ug-img0001.jpg "Western Blot: Complement C3 Antibody (X1) [H00000718-M11-100ug] - Detection against Immunogen (37.95 kDa).")