![Immunocytochemistry/Immunofluorescence: Collagen IV Antibody (1043) [NBP1-97716] - hS/PCs sequentially secrete basement membrane proteins during microstructure reorganization and function. Encapsulated hS/PCs in HA-PEGDA hydrogels initially produce collagen IV (green) (D-F) even at the single-cell state. Scale bar = 20 um in all confocal micrographs. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-1043-Immunocytochemistry-Immunofluorescence-NBP1-97716-img0008.jpg "Immunocytochemistry/Immunofluorescence: Collagen IV Antibody (1043) [NBP1-97716] - hS/PCs sequentially secrete basement membrane proteins during microstructure reorganization and function. Encapsulated hS/PCs in HA-PEGDA hydrogels initially produce collagen IV (green) (D-F) even at the single-cell state. Scale bar = 20 um in all confocal micrographs. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 1043 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Immunogen | Derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta. |
| Specificity | This antibody recognizes Collagen IV in the extracellular - basement membranes of normal and pathological human tissues. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | COL4A1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is suitable for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications. This antibody is CyTOF ready. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2 |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
| Publications using NBP1-97716 | Applications | Species |
|---|---|---|
| Irizarry BA Morphology, Propagation, and Toxicity of Human-Derived Amyloid-? Fibrils Thesis 2023-01-01 (IHC, Human) | IHC | Human |
| Liu S, Li B, Liu N et al. Multi-omics analysis of human tendon adhesion reveals ACKR1 migrates macrophages involve in endogenous regeneration Research Square 2023-10-30 (IHC, Human) | IHC | Human |
| Wu D, Witt RL, Harrington DA, Farach-Carson MC Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated alpha1 beta1 Integrin-Mediated Motility Front Cell Dev Biol 2019-10-16 [PMID: 31750298] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen IV Antibody (NBP1-97716)Find related products by research area.
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| Gene Symbol | COL4A1 |
![Immunohistochemistry-Frozen: Collagen IV Antibody (1043) [NBP1-97716] - Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney.](http://images.novusbio.com/fullsize/Collagen-IV-alpha-1-Antibody-1043-Immunohistochemistry-Frozen-NBP1-97716-img0006.jpg "Immunohistochemistry-Frozen: Collagen IV Antibody (1043) [NBP1-97716] - Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney.")
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![Immunohistochemistry-Frozen: Collagen IV Antibody (1043) [NBP1-97716] - Frozen section of Human colon](http://images.novusbio.com/fullsize/Collagen-IV-alpha-1-Antibody-1043-Immunohistochemistry-Frozen-NBP1-97716-img0005.jpg "Immunohistochemistry-Frozen: Collagen IV Antibody (1043) [NBP1-97716] - Frozen section of Human colon")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")