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Collagen II Antibody (5B2.5) - Azide and BSA Free

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Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. Image from the standard format of t
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
5B2.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-844AF350
Alexa Fluor 405
NB600-844AF405
Alexa Fluor 488
NB600-844AF488
Alexa Fluor 532
NB600-844AF532
Alexa Fluor 594
NB600-844AF594
Alexa Fluor 647
NB600-844AF647
Alexa Fluor 700
NB600-844AF700
Alexa Fluor 750
NB600-844AF750
Allophycocyanin
NB600-844APC
Biotin
NB600-844B
DyLight 350
NB600-844UV
DyLight 405
NB600-844V
DyLight 488
NB600-844G
DyLight 550
NB600-844R
DyLight 594
NB600-844DL594
DyLight 650
NB600-844C
DyLight 680
NB600-844FR
DyLight 755
NB600-844IR
FITC
NB600-844F
HRP
NB600-844H
Janelia Fluor 549
NB600-844JF549
Janelia Fluor 646
NB600-844JF646
PE
NB600-844PE
PerCP
NB600-844PCP
Unconjugated
NB600-844
mFluor Violet 450 SE
NB600-844MFV450
mFluor Violet 500 SE
NB600-844MFV500
mFluor Violet 610 SE
NB600-844MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-844
BSA Free
NB600-844
BSA Free
NB600-844SS
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Collagen II Antibody (5B2.5) - Azide and BSA Free Summary

Immunogen
A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Localization
Cartilage matrix
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL2A1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry reported in scientific literature (PMID 22558278; 24242000)
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 22558278)
  • Immunohistochemistry-Paraffin
  • Western Blot 1-5ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Collagen II Antibody (5B2.5) - Azide and BSA Free

  • Alpha-1 type II collagen
  • ANFH
  • AOM
  • cartilage collagen
  • Chondrocalcin
  • COL11A3
  • COL2A1
  • Collagen 2
  • collagen alpha-1(II) chain
  • Collagen II
  • collagen II, alpha-1 polypeptide
  • collagen type 2
  • collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
  • collagen, type II, alpha 1
  • Collagen-2
  • MGC131516
  • SEDC

Background

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL2A1