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Rat COL1A2 ELISA Kit (Colorimetric)

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ELISA: Rat COL1A2 ELISA Kit (Colorimetric) [NBP2-75847] - Samples were spiked with high concentrations of Rat COL1A2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat COL1A2 ELISA Kit (Colorimetric) [NBP2-75847] - Samples were spiked with high concentrations of Rat COL1A2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Rat COL1A2 ELISA Kit (Colorimetric) [NBP2-75847] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

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Rat COL1A2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat COL1a2 in samples. No significant cross-reactivity or interference between Rat COL1a2 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.9%
Intra-Assay
CV% < 4.78%
Spike Recovery
85-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
COL1A2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat COL1A2 ELISA Kit (Colorimetric)

  • alpha 2(I)-collagen
  • Alpha-2 type I collagen
  • collagen alpha-2(I) chain
  • collagen I, alpha-2 polypeptide
  • collagen of skin, tendon and bone, alpha-2 chain
  • collagen, type I, alpha 2
  • OI4
  • osteogenesis imperfecta type IV
  • type I procollagen

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Rat COL1A2 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol COL1A2