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CLTB Antibody (4B12-1E3)

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Western Blot: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of CLTB expression in transfected 293T cell line by CLTB monoclonal antibody (M01), clone 4B12-1E3.Lane 1: CLTB transfected lysate(23.2 KDa).Lane 2: Non-transfected lysate.
Western Blot: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of CLTB expression in transfected 293T cell line by CLTB monoclonal antibody (M01), clone 4B12-1E3.Lane 1: CLTB transfected lysate(23.2 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of monoclonal antibody to CLTB on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of monoclonal antibody to CLTB on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 2 ...read more
Western Blot: CLTB Antibody (4B12-1E3) [H00001212-M01] - CLTB monoclonal antibody (M01), clone 4B12-1E3 Analysis of CLTB expression in MCF-7.
ELISA: CLTB Antibody (4B12-1E3) [H00001212-M01] - Detection limit for recombinant GST tagged CLTB is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4B12-1E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CLTB Antibody (4B12-1E3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CLTB (AAH06457, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR
Localization
Cytoplasmic
Specificity
CLTB - clathrin, light polypeptide (Lcb)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CLTB
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publication using H00001212-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLTB Antibody (4B12-1E3)

  • clathrin light chain B
  • clathrin, light chain (Lcb)
  • clathrin, light chain B
  • clathrin, light polypeptide (Lcb)
  • Lcb

Background

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CLTB Antibody
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H00001212-M01
CLTB Antibody (4B12-1E3)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC

Publications for CLTB Antibody (H00001212-M01)(1)


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Bioinformatics

Gene Symbol CLTB
Entrez
OMIM
Uniprot