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Clathrin light chain + heavy chain Antibody

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Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Clathrin light chain + heavy chain Antibody Summary

Immunogen
Peptide with sequence CDFNPKSSKQAKD corresponding to internal region according to NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1.
Epitope
internal region
Specificity
This antibody is expected to recognize all reported isoforms of light chains a and b.
Predicted Species
Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLTA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
Western blot: Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen.
Publications
Read Publications using
NBP1-05035 in the following applications:

  • WB
    1 publication

Reactivity Notes

Use in Rat reported in scientific literature (PMID:24876496).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Clathrin light chain + heavy chain Antibody

  • clathrin light chain A
  • clathrin, light chain A
  • Lca
  • light polypeptide (Lca)

Background

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Transcript Variant: This variant (1) lacks alternate in-frame exons, compared to variant 2, resulting in a shorter protein (isoform a), compared to isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Clathrin light chain + heavy chain Antibody (NBP1-05035)(3)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol CLTA
Entrez
Uniprot