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CLIP170 Antibody (CL4851)

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Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144] - Analysis in human cell line HDLM-2 and human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of A549 cells using the Anti-CLIP1 monoclonal antibody, showing specific staining in microtubule ends in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human kidney shows moderate to strong cytoplasmic immunoreactivity in renal tubules.
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human cerebellum shows moderate cytoplasmic immunoreactivity in a subset of neuronal cells.
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblast.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL4851
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CLIP170 Antibody (CL4851) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:KNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDET
Predicted Species
Mouse (97%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLIP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:10000 - 1:20000
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CLIP170 Antibody (CL4851)

  • CAP-GLY domain containing linker protein 1
  • CLIP
  • CLIP170
  • CLIP-170Reed-Sternberg intermediate filament-associated protein
  • CYLN1MGC131604
  • cytoplasmic linker 1
  • Cytoplasmic linker protein 1
  • Cytoplasmic linker protein 170 alpha-2
  • Reed-Steinberg cell-expressed intermediate filament-associated protein
  • restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
  • Restin
  • RSNCAP-Gly domain-containing linker protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLIP1