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EB2 Antibody (OTI1F3)

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Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.
Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: EB2 Antibody (1F3) [NBP2-00714] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.
Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Human pancreas tissue using anti-EB2 mouse monoclonal antibody.
Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EB2 antibody, and then analyzed by ...read more
Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EB2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EB2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EB2 mouse monoclonal antibody.
Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Hela cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Jurkat cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.81 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71650AF350
Alexa Fluor 405
NBP2-71650AF405
Alexa Fluor 488
NBP2-71650AF488
Alexa Fluor 532
NBP2-71650AF532
Alexa Fluor 594
NBP2-71650AF594
Alexa Fluor 647
NBP2-71650AF647
Alexa Fluor 700
NBP2-71650AF700
Alexa Fluor 750
NBP2-71650AF750
Allophycocyanin
NBP2-71650APC
Allophycocyanin/Cy7
NBP2-71650APCCY7
Biotin
NBP2-71650B
DyLight 350
NBP2-71650UV
DyLight 405
NBP2-71650V
DyLight 488
NBP2-71650G
DyLight 550
NBP2-71650R
DyLight 594
NBP2-71650DL594
DyLight 650
NBP2-71650C
DyLight 680
NBP2-71650FR
DyLight 755
NBP2-71650IR
FITC
NBP2-71650F
HRP
NBP2-71650H
Janelia Fluor 549
NBP2-71650JF549
Janelia Fluor 646
NBP2-71650JF646
PE
NBP2-71650PE
PE/Atto594
NBP2-71650PEATT594
PE/Cy5.5
NBP2-71650PECY55
PE/Cy7
NBP2-71650PECY7
PerCP
NBP2-71650PCP
mFluor Violet 450 SE
NBP2-71650MFV450
mFluor Violet 500 SE
NBP2-71650MFV500
mFluor Violet 610 SE
NBP2-71650MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71650
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EB2 Antibody (OTI1F3) Summary

Immunogen
Full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPRE2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.81 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EB2 Antibody (OTI1F3)

  • APC-binding protein EB2
  • EB1
  • EB2APC-binding protein EB1
  • End-binding protein 2
  • microtubule-associated protein, RP/EB family, member 2
  • RP1microtubule-associated protein RP/EB family member 2
  • T-cell activation protein, EB1 family

Background

The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAPRE2
Entrez