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CLEC3B/Tetranectin Antibody (805035) [Unconjugated]

 
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Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more
Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N
Clone
805035
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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MAB5170-SP
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CLEC3B/Tetranectin Antibody (805035) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC3B/Tetranectin
Glu22-Val202
Accession # P05452
Specificity
Detects human CLEC3B/Tetranectin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC3B
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC3B/Tetranectin Antibody (805035) [Unconjugated]

  • CLEC3B
  • C-type lectin domain family 3 member B
  • C-type lectin domain family 3, member B
  • DKFZp686H17246
  • Plasminogen kringle 4-binding protein
  • TETN
  • tetranectin (plasminogen binding protein)
  • Tetranectin
  • TNA
  • TNAtetranectin (plasminogen-binding protein)
  • TNtetranectin

Background

CLEC3B (C-type lectin domain family 3-member B; also Tetranectin) is a 20 kDa, secreted member of the C type lectin superfamily. It is produced by multiple cell types including monocytes, neutrophils, fibroblasts, hepatocytes and various endocrine cells. Although named Tetranectin, CLEC3B is actually a nondisulfide linked homotrimer. It binds to plasminogen, sulfated polysaccharides, and fibrin. Human CLEC3B precursor is 202 amino acids (aa) in length. It contains a signal sequence (aa 1-21), a coiled coil region that mediates trimerization (aa 26-52) and a C type lectin domain (aa 77-198). CLEC3B contains O-linked glycosylation. Mature human CLEC3B is 81% aa identical to mouse CLEC3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLEC3B
Uniprot