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Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4)

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Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). Predicted band size: 192kDa. Observed band size: 192kDa.
Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). ...read more
Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Immunohistochemistry of Clathrin Heavy Chain 1/CHC17 in paraffin-embedded Human colon cancer tissue using NBP3-19848 at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
S02-6C4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

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Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Clathrin Heavy Chain 1/CHC17 (Uniprot # Q00610)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CLTC
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4)

  • CHC
  • CHC17
  • Clathrin Heavy Chain 1
  • Clathrin heavy chain on chromosome 17
  • clathrin, heavy chain (Hc)
  • clathrin, heavy chain
  • clathrin, heavy polypeptide (Hc)
  • clathrin, heavy polypeptide-like 2
  • CLH17
  • CLH-17
  • CLTC
  • CLTCL2
  • Hc
  • KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLTC