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CISD1 Antibody (OTI4G3)

Images

 
Western Blot: CISD1 Antibody (4G3) [NBP2-02321] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: CISD1 Antibody (4G3) [NBP2-02321] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1.
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Kidney tissue using anti-CISD1mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CISD1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CISD1 Antibody (4G3) [NBP2-02321] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CISD1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.68 mg/ml

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CISD1 Antibody (OTI4G3) Summary

Immunogen
Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CISD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.68 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CISD1 Antibody (OTI4G3)

  • C10orf70
  • CDGSH iron sulfur domain 1
  • CDGSH iron sulfur domain-containing protein 1
  • CDGSH iron-sulfur domain-containing protein 1
  • chromosome 10 open reading frame 70
  • MDS029
  • MGC14684
  • mitoNEET
  • ZCD1
  • zinc finger CDGSH-type domain 1
  • zinc finger, CDGSH-type domain 1

Background

CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CISD1
Entrez