![Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanCISD1 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysat](http://images.novusbio.com/fullsize/CISD1-Antibody-1A8-Western-Blot-NBP2-22567-img0003.jpg "Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanCISD1 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysat")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow |
| Clone | 1A8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human CISD1 (32-108aa) purified from E. coli |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CISD1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: CISD1 Antibody (1A8) [NBP2-22567] - Analysis of CISD1 in THP-1 cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/CISD1-Antibody-1A8-Flow-Cytometry-NBP2-22567-img0002.jpg "Flow Cytometry: CISD1 Antibody (1A8) [NBP2-22567] - Analysis of CISD1 in THP-1 cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).")
![Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/CISD1-Antibody-1A8-Western-Blot-NBP2-22567-img0001.jpg "Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.")
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