![Western Blot: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Western Blot Analysis of MCF-7 & PC3 cell lysates using Chp2 Mouse Monoclonal Antibody (CPTC-CHP2-1).](http://images.novusbio.com/fullsize/Chp2-Antibody-CPTC-CHP2-1-Western-Blot-NBP3-07819-img0002.jpg "Western Blot: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Western Blot Analysis of MCF-7 & PC3 cell lysates using Chp2 Mouse Monoclonal Antibody (CPTC-CHP2-1).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CPTC-CHP2-1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08694) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human full-length Chp2 protein (Uniprot: O43745) |
| Localization | Nucleus, Cytoplasm |
| Isotype | IgG2c Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHP2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunocytochemistry__Immunofluorescence_23325.jpg)
![Neutralization Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Block_Neutralize_8975.jpg)
![Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunohistochemistry_9333.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Immunofluorescence Analysis of human MCF-7 cells labeling Chp2 with Chp2 Mouse Monoclonal Antibody (CPTC-Chp2-1) followed by Goat anti- Mouse IgG-CF488 (Green).The nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/Chp2-Antibody-CPTC-CHP2-1-Immunocytochemistry-Immunofluorescence-NBP3-07819-img0001.jpg "Immunocytochemistry/Immunofluorescence: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Immunofluorescence Analysis of human MCF-7 cells labeling Chp2 with Chp2 Mouse Monoclonal Antibody (CPTC-Chp2-1) followed by Goat anti- Mouse IgG-CF488 (Green).The nuclear counterstain is RedDot (Red)")
![N/A alpha-Defensin 1 [Biotin]](https://images.novusbio.com/images2/DATA_alphaDefensin_1_DY819805_ELISA_2534.jpg)
![Western Blot NFATC3/NFAT4 Antibody [Unconjugated]](https://images.novusbio.com/images2/NFATC3_AF5834_Western_Blot_5863.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Western Blot Contactin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Contactin-3_AF5539_Western_Blot_5751.jpg)
![Immunocytochemistry Contactin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Contactin-3_AF5539_Immunocytochemistry_11752.jpg)
