Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) followed ...read more
Contactin-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) at 10 µg/mL for 3 hours at ...read more
Detects human, mouse, and rat Contactin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Contactin-5 and recombinant mouse Contactin-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTN3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Contactin-3 Antibody [Unconjugated]
BIG-1
CNTN3
contactin 3 (plasmacytoma associated)
Contactin3
Contactin-3
PANG
PCS
Background
Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. Its exact function is unclear, but it likely mediates cell-cell interaction and may promote neurite outgrowth. Human Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG like domains (aa 26-593), followed by four fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, human Contactin-3 shares 92% aa identity with mouse Contactin‑3.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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