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CHD4 Antibody (CHD4 3F2/4) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready, KO
Clone
CHD4 3F2/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50163AF350
Alexa Fluor 405
NBP2-50163AF405
Alexa Fluor 488
NBP2-50163AF488
Alexa Fluor 532
NBP2-50163AF532
Alexa Fluor 594
NBP2-50163AF594
Alexa Fluor 647
NBP2-50163AF647
Alexa Fluor 700
NBP2-50163AF700
Alexa Fluor 750
NBP2-50163AF750
Allophycocyanin
NBP2-50163APC
Biotin
NBP2-50163B
CoraFluor 1
NBP2-50163CL1
DyLight 350
NBP2-50163UV
DyLight 405
NBP2-50163V
DyLight 488
NBP2-50163G
DyLight 550
NBP2-50163R
DyLight 594
NBP2-50163DL594
DyLight 650
NBP2-50163C
DyLight 680
NBP2-50163FR
DyLight 755
NBP2-50163IR
FITC
NBP2-50163F
HRP
NBP2-50163H
Janelia Fluor 525
NBP2-50163JF525
Janelia Fluor 585
NBP2-50163JF585
Janelia Fluor 635
NBP2-50163JF635
Janelia Fluor 646
NBP2-50163JF646
Janelia Fluor 669
NBP2-50163JF669
PE
NBP2-50163PE
PerCP
NBP2-50163PCP
Unconjugated
NBP2-50163
mFluor Violet 450 SE
NBP2-50163MFV450
mFluor Violet 500 SE
NBP2-50163MFV500
mFluor Violet 610 SE
NBP2-50163MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-50163
BSA Free
NBP2-50163
BSA Free
NBP2-50163-0.025ml
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CHD4 Antibody (CHD4 3F2/4) [Janelia Fluor® 549] Summary

Immunogen
Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CHD4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CHD4 Antibody (CHD4 3F2/4) [Janelia Fluor® 549]

  • ATP-dependent helicase CHD4
  • CHD-4
  • chromodomain helicase DNA binding protein 4
  • chromodomain-helicase-DNA-binding protein 4
  • DKFZp686E06161
  • EC 3.6.1
  • EC 3.6.4.12
  • Mi-2 autoantigen 218 kDa protein
  • Mi-2b
  • Mi2-BETA

Background

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHD4