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CHD4 Antibody (CHD4 3F2/4) [DyLight 650]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready, KO
Clone
CHD4 3F2/4
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50163AF350
Alexa Fluor 405
NBP2-50163AF405
Alexa Fluor 488
NBP2-50163AF488
Alexa Fluor 532
NBP2-50163AF532
Alexa Fluor 594
NBP2-50163AF594
Alexa Fluor 647
NBP2-50163AF647
Alexa Fluor 700
NBP2-50163AF700
Alexa Fluor 750
NBP2-50163AF750
Allophycocyanin
NBP2-50163APC
Biotin
NBP2-50163B
CoraFluor 1
NBP2-50163CL1
DyLight 350
NBP2-50163UV
DyLight 405
NBP2-50163V
DyLight 488
NBP2-50163G
DyLight 550
NBP2-50163R
DyLight 594
NBP2-50163DL594
DyLight 680
NBP2-50163FR
DyLight 755
NBP2-50163IR
FITC
NBP2-50163F
HRP
NBP2-50163H
Janelia Fluor 525
NBP2-50163JF525
Janelia Fluor 549
NBP2-50163JF549
Janelia Fluor 585
NBP2-50163JF585
Janelia Fluor 635
NBP2-50163JF635
Janelia Fluor 646
NBP2-50163JF646
Janelia Fluor 669
NBP2-50163JF669
PE
NBP2-50163PE
PerCP
NBP2-50163PCP
Unconjugated
NBP2-50163
mFluor Violet 450 SE
NBP2-50163MFV450
mFluor Violet 500 SE
NBP2-50163MFV500
mFluor Violet 610 SE
NBP2-50163MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-50163
BSA Free
NBP2-50163
BSA Free
NBP2-50163-0.025ml
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CHD4 Antibody (CHD4 3F2/4) [DyLight 650] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CHD4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CHD4 Antibody (CHD4 3F2/4) [DyLight 650]

  • ATP-dependent helicase CHD4
  • CHD-4
  • chromodomain helicase DNA binding protein 4
  • chromodomain-helicase-DNA-binding protein 4
  • DKFZp686E06161
  • EC 3.6.1
  • EC 3.6.4.12
  • Mi-2 autoantigen 218 kDa protein
  • Mi-2b
  • Mi2-BETA

Background

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHD4