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CHD4 Antibody (CHD4 3F2/4) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready, KO
Clone
CHD4 3F2/4
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

Order Details

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CHD4 Antibody (CHD4 3F2/4) [Alexa Fluor® 594] Summary

Immunogen
Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CHD4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CHD4 Antibody (CHD4 3F2/4) [Alexa Fluor® 594]

  • ATP-dependent helicase CHD4
  • CHD-4
  • chromodomain helicase DNA binding protein 4
  • chromodomain-helicase-DNA-binding protein 4
  • DKFZp686E06161
  • EC 3.6.1
  • EC 3.6.4.12
  • Mi-2 autoantigen 218 kDa protein
  • Mi-2b
  • Mi2-BETA

Background

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHD4