CHD4 Antibodies
We offer CHD4 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each CHD4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CHD4 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the CHD4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CHD4 polyclonal antibodies and browse our CHD4 monoclonal antibody catalog.
Alternate Names for CHD4 Antibodies
anti-CHD4 antibody, anti-ATP-dependent helicase CHD4 antibody, anti-CHD-4 antibody, anti-chromodomain helicase DNA binding protein 4 antibody, anti-chromodomain-helicase-DNA-binding protein 4 antibody, anti-DKFZp686E06161 antibody, anti-EC 3.6.1 antibody, anti-EC 3.6.4.12 antibody, anti-Mi-2 autoantigen 218 kDa protein antibody, anti-Mi-2b antibody, anti-Mi2-BETA antibody
18 Results for "chd4" in Primary Antibodies
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Azide-and-BSA-Free-Western-Blot-NBP3-08830-img0003.jpg "Western Blot: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08830-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD4 Antibody (3F2/4) - Azide and BSA Free [NBP3-08830] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08830-img0002.jpg)
![Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Western-Blot-NBP3-07505-img0003.jpg "Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody (3F2/4) [NBP3-07505] - Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Immunocytochemistry-Immunofluorescence-NBP3-07505-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD4 Antibody (3F2/4) [NBP3-07505] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Immunohistochemistry-Paraffin-NBP3-07505-img0002.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0009.jpg "Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Immunohistochemistry-Paraffin-H00001108-M01-img0006.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0008.jpg)
![Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Sandwich-ELISA-H00001108-M01-img0007.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-NB100-57521-img0003.jpg)
![Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabbit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CHD4-Antibody-BLR066G-Western-Blot-NBP2-77358-img0002.jpg "Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabbit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/CHD4-Antibody-BLR066G-Western-Blot-NBP2-77358-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody (BLR066G) [NBP2-77358] - Detection of human CHD4 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358) and rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520, NB100-57521).](http://images.novusbio.com/fullsize/CHD4-Antibody-BLR066G-Immunoprecipitation-NBP2-77358-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP1-82651] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82651-img0004.jpg "Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP1-82651] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP1-82651-img0001.jpg)
![Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57520-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.")
![Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NBP2-99099-img0002.jpg "Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99099] - Immunofluorescence staining of CHD4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CHD4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99099-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99098] - Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CHD4 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99098-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99098] - Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CHD4 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP2-99098] - Immunochemical staining of human CHD4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-99098-img0002.jpg)
![Western Blot: CHD4 Antibody (SR1411) [NBP3-21737] - Western blot analysis using NBP3-21737 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21737_rabbit-chd4-mab-sr1411-57202316582186.jpg "Western Blot: CHD4 Antibody (SR1411) [NBP3-21737] - Western blot analysis using NBP3-21737 in 293T cell lysate.")