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CHD1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining in human testis and skeletal muscle tissues.. Corresponding CHD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human placenta shows moderate to strong nuclear positivity.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CHD1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: QESQQNSDQNSNLNPHVIRNPDVERLKENTNHDDSSRDSYSSDRHLTQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHRDWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSL
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CHD1 Protein (NBP2-14478PEP)

Reactivity Notes

Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CHD1 Antibody

  • ATP-dependent helicase CHD1
  • CHD1
  • CHD-1
  • chromodomain helicase DNA binding protein 1
  • chromodomain-helicase-DNA-binding protein 1
  • DKFZp686E2337
  • EC 3.6.1
  • EC 3.6.4.12

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CHD1 Antibody (NBP2-14478) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CHD1 Products

Array NBP2-14478

Research Areas for CHD1 Antibody (NBP2-14478)

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Blogs on CHD1

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Bioinformatics

Gene Symbol CHD1