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Centrin 1 Antibody

Images

 
Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 ug of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 ...read more
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.

Product Details

Summary
Reactivity Hu, Bv, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Centrin 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary.
Predicted Species
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CETN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%), Chicken (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Centrin 1 Antibody

  • calcium binding protein
  • Caltractin isoform 2
  • caltractin
  • CEN1centrin-1
  • centrin, EF-hand protein, 1
  • CETN
  • EF-hand protein

Background

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CETN1
Uniprot