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CDYL Antibody

Images

 
Western Blot: CDYL Antibody [NBP2-34011] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: CDYL Antibody [NBP2-34011] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Analysis in human placenta and pancreas tissues. Corresponding CDYL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CDYL Antibody [NBP2-34011] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons and glial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CDYL Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PVKSRTAVDGFQSESPEKLDPVEQGQEDTVAPEVAAEKPVGALLGPGAERARMGSRPRIHP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDYL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CDYL Protein (NBP2-34011PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CDYL Antibody

  • CDYL1bA620A17.2 (chromodomain protein, Y chromosome-like)
  • CDY-like
  • CDY-like, autosomal
  • chromodomain protein, Y chromosome-like
  • chromodomain protein, Y-like
  • chromodomain Y-like protein
  • DKFZP586C1622
  • EC 2.3.1.48
  • MGC131936
  • testis-specific chromodomain Y-like protein

Background

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CDYL
Uniprot