CDC7 Antibody (SR2732) Summary
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
A synthesized peptide derived from human CDC7 (Uniprot #: O00311) |
Specificity |
Detects endogenous levels of total CDC7 |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
CDC7 |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:100
- Western Blot 1:1000-1:2000
|
Theoretical MW |
70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4), 150mM NaCl, 50% glycerol |
Preservative |
0.02% Sodium Azide |
Purity |
Affinity purified |
Alternate Names for CDC7 Antibody (SR2732)
Background
DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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