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CDC7 Antibody (SR2732)

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Western Blot: CDC7 Antibody (SR2732) [NBP3-27710] - Western blot analysis of extracts from HeLa cells

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow
Clone
SR2732
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CDC7 Antibody (SR2732) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human CDC7 (Uniprot #: O00311)
Specificity
Detects endogenous levels of total CDC7
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDC7
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Western Blot 1:1000-1:2000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 150mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CDC7 Antibody (SR2732)

  • CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
  • CDC7 cell division cycle 7 (S. cerevisiae)
  • CDC7
  • CDC7L1
  • CDC7L1MGC117361
  • CDC7-related kinase
  • cell division cycle 7 (S. cerevisiae)
  • cell division cycle 7 homolog (S. cerevisiae)
  • cell division cycle 7-like protein 1
  • cell division cycle 7-related protein kinase
  • EC 2.7.11.1
  • HsCdc7
  • Hsk1
  • huCdc7
  • MGC126237
  • MGC126238

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDC7