CD47 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IF, IHC, Immunohistochemistry, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using AF1866	Applications	Species
Moon, S;Han, S;Jang, IH;Ryu, J;Rha, MS;Cho, HJ;Yoon, SS;Nam, KT;Kim, CH;Park, MS;Seong, JK;Lee, WJ;Yoon, JH;Chung, YW;Ryu, JH; Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection Nature communications 2024-04-30 [PMID: 38693120] (Immunohistochemistry, Western Blot, Mouse)	Immunohistochemistry, Western Blot	Mouse
Tang, Z;Zhong, MC;Qian, J;Galindo, CC;Davidson, D;Li, J;Zhao, Y;Hui, E;Veillette, A; CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity Nature immunology 2023-11-09 [PMID: 37945822] (Immunoprecipitation, Western Blot, Mouse)	Immunoprecipitation, Western Blot	Mouse
Linyong Du, Zhipeng Su, Silu Wang, Ying Meng, Fei Xiao, Daqian Xu et al. EGFR‐Induced and c‐Src‐Mediated CD47 Phosphorylation Inhibits TRIM21‐Dependent Polyubiquitylation and Degradation of CD47 to Promote Tumor Immune Evasion Advanced Science 8/4/2023 [PMID: 37541303]
Pizzato, HA;Alonso-Guallart, P;Woods, J;Johannesson, B;Connelly, JP;Fehniger, TA;Atkinson, JP;Pruett-Miller, SM;Monsma, FJ;Bhattacharya, D; Engineering Human Pluripotent Stem Cell Lines to Evade Xenogeneic Transplantation Barriers bioRxiv : the preprint server for biology 2023-06-29 [PMID: 37425790] (IHC, Mouse)	IHC	Mouse
Singla B, Aithbathula RV, Pervaiz N et al. CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis Arteriosclerosis, thrombosis, and vascular biology 7/1/2023 [PMID: 37259865] Details: IHC Dilution: 1:100
Paul A. Mueller, Yoko Kojima, Katherine T. Huynh, Richard A. Maldonado, Jianqin Ye, Hagai Tavori et al. Macrophage LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Is Required for the Effect of CD47 Blockade on Efferocytosis and Atherogenesis—Brief Report Arteriosclerosis, Thrombosis, and Vascular Biology 1/1/2022 [PMID: 34758632]
X Ding, J Wang, M Huang, Z Chen, J Liu, Q Zhang, C Zhang, Y Xiang, K Zen, L Li Loss of microglial SIRP&alpha promotes synaptic pruning in preclinical models of neurodegeneration Nature Communications, 2021-04-01;12(1):2030. 2021-04-01 [PMID: 33795678] (Western Blot, Mouse)	Western Blot	Mouse
T Lerbs, L Cui, ME King, T Chai, C Muscat, L Chung, R Brown, K Rieger, T Shibata, G Wernig CD47 prevents the elimination of diseased fibroblasts in scleroderma JCI Insight, 2020-08-20;5(16):. 2020-08-20 [PMID: 32814713] (ICC, IHC, Mouse)	ICC, IHC	Mouse
V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo Mucosal Immunol, 2020-06-19;0(0):. 2020-06-19 [PMID: 32561828] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
M Reed, AC Luissint, V Azcutia, S Fan, MN O'Leary, M Quiros, J Brazil, A Nusrat, CA Parkos Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo Nat Commun, 2019-11-01;10(1):5004. 2019-11-01 [PMID: 31676794] (IF, Western Blot, Mouse)	IF, Western Blot	Mouse
CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis Nature, 2016-07-20;0(0):. 7/20/2016 [PMID: 27437576]
Vermeer D, Spanos W, Vermeer P, Bruns A, Lee K, Lee J Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer. Int J Cancer, 2013-02-12;133(1):120-9. 2013-02-12 [PMID: 23292955] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	04/03/2020	View Summary Application Western Blot Sample Tested 4T1 cells whole cell lysate,EMT6 cells Species Mouse Lot KBA0216121

Array AF1866

• CD47 Antibodies
• CD47 Lysates
• CD47 Proteins
• CD47 Proteins and Enzymes
• CD47 ELISA