![Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (T200/797).](http://images.novusbio.com/fullsize/CD45RO-Antibody-T200-797-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54315-img0001.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (T200/797).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | T200/797 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44860). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Human T-lymphocytes (Uniprot: P08575) |
| Localization | Cell surface |
| Marker | T-Cell Marker |
| Specificity | Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTPRC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 182 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD45 Antibody (NBP2-54315)Find related products by research area.
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| Gene Symbol | PTPRC |
![Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RO-Antibody-T200-797-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54315-img0002.jpg "Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).")
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