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CD45RO Antibody (T200/797) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
T200/797
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54315AF350
Alexa Fluor 405
NBP2-54315AF405
Alexa Fluor 488
NBP2-54315AF488
Alexa Fluor 532
NBP2-54315AF532
Alexa Fluor 647
NBP2-54315AF647
Alexa Fluor 700
NBP2-54315AF700
Alexa Fluor 750
NBP2-54315AF750
Allophycocyanin
NBP2-54315APC
Allophycocyanin/Cy7
NBP2-54315APCCY7
Biotin
NBP2-54315B
CoraFluor 1
NBP2-54315CL1
DyLight 350
NBP2-54315UV
DyLight 405
NBP2-54315V
DyLight 488
NBP2-54315G
DyLight 550
NBP2-54315R
DyLight 594
NBP2-54315DL594
DyLight 650
NBP2-54315C
DyLight 680
NBP2-54315FR
DyLight 755
NBP2-54315IR
FITC
NBP2-54315F
HRP
NBP2-54315H
Janelia Fluor 525
NBP2-54315JF525
Janelia Fluor 549
NBP2-54315JF549
Janelia Fluor 585
NBP2-54315JF585
Janelia Fluor 635
NBP2-54315JF635
Janelia Fluor 646
NBP2-54315JF646
Janelia Fluor 669
NBP2-54315JF669
PE
NBP2-54315PE
PE/Atto594
NBP2-54315PEATT594
PE/Cy5.5
NBP2-54315PECY55
PE/Cy7
NBP2-54315PECY7
PerCP
NBP2-54315PCP
Unconjugated
NBP2-44860-0.1mg
mFluor Violet 450 SE
NBP2-54315MFV450
mFluor Violet 500 SE
NBP2-54315MFV500
mFluor Violet 610 SE
NBP2-54315MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44860-0.1mg
IHC-Prediluted
NBP2-48465
Azide and BSA Free
NBP2-54315-100ug
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CD45RO Antibody (T200/797) [Alexa Fluor® 594] Summary

Immunogen
Human T-lymphocytes (Uniprot: P08575)
Localization
Cell surface
Marker
T-Cell Marker
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD45RO Antibody (T200/797) [Alexa Fluor® 594]

  • B220
  • CD45 antigen
  • CD45R
  • CD45RO
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CD45RO Antibody (NBP2-54315AF594). (Showing 1 - 1 of 1 FAQ).

  1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
    • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPRC