CD45 Antibody (2B11 + PD7/26) - Azide and BSA Free

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, IHC-P.

Showing Publications 1 - 8 of 8.

Publications using NBP2-34528	Applications	Species
Casper W.F. van Eijck, Francisco X. Real, Núria Malats, Disha Vadgama, Thierry P.P. van den Bosch, Michail Doukas, Casper H.J. van Eijck, Dana A.M. Mustafa GATA6 identifies an immune-enriched phenotype linked to favorable outcomes in patients with pancreatic cancer undergoing upfront surgery Cell Reports Medicine 2024-05-10 [PMID: 38733987]
Huang H, Li N, Liang Y et al. Multi-omics analyses reveal spatial heterogeneity in primary and metastatic oesophageal squamous cell carcinoma Clinical and translational medicine 2023-11-01 [PMID: 38009315] (IHC-P, Human)	IHC-P	Human
Lovewell RR, Hong J, Kundu S et al. LAIR-1 agonism as a therapy for acute myeloid leukemia The Journal of clinical investigation 2023-11-15 [PMID: 37966113] (IHC-P, Mouse)	IHC-P	Mouse
Ragazzini R, Boeing S, Zanieri L et al. Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus Developmental cell 2023-08-28 [PMID: 37652013]
Caputo A, Vipparthi K, Bazeley P et al. Alterations in the preneoplastic breast microenvironment ofBRCA1/2mutation carriers revealed by spatial transcriptomics bioRxiv 2023-05-25 [PMID: 37292816] (IHC-P, Human)	IHC-P	Human
KrAmer B, Nalin A, Ma F et al. Single-cell RNA sequencing identifies a population of human liver-type ILC1s Cell Reports 2023-01-01 [PMID: 36640314] (FLOW, Human)	FLOW	Human
Smith KD, Prince DK, Henriksen KJ Et al. Digital spatial profiling of collapsing glomerulopathy Kidney Int 2022-03-01 [PMID: 35227689] (IHC-P, Human) Details: Citation using the Azide and BSA Free version of this antibody.	IHC-P	Human
D Phillips, M Matusiak, BR Gutierrez, SS Bhate, GL Barlow, S Jiang, J Demeter, KS Smythe, RH Pierce, SP Fling, N Ramchurren, MA Cheever, Y Goltsev, RB West, MS Khodadoust, YH Kim, CM Schürch, GP Nolan Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma Nature Communications, 2021-11-18;12(1):6726. 2021-11-18 [PMID: 34795254]

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	Immunofluorescence - paraffin	Human	11/18/2018	View Summary Application Other Sample Tested human tonsil Species Human Lot I don't have it any more Comments Comments pH 9 antigen retrieval

Array NBP2-34528

• CD45 Antibodies
• CD45 Lysate
• CD45 Proteins
• CD45 Proteins and Enzymes