CD40 Ligand/TNFSF5 Antibody (40804) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD40 Ligand (CD40L), now renamed TNFSF5 but also known as CD154, TRAP and gp39, is a 34‑39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1‑3). Human CD40L is 261 amino acids (aa) in length and consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane segment, and a 215 aa extracellular region that consists of multiple beta -strands and one N‑linked glycosylation site (4, 5). Although carbohydrates are present, they are not necessary for activity (6). As with other TNF superfamily members, CD40L will exist as a trimer, both as a membrane bound and soluble form (6‑8). The soluble form is 18 kDa in size and about 150 aa in length, and arises from intracellular proteolytic processing. As a trimer, the soluble form is bioactive (7‑9). Multiple mutations and alternate splice forms of CD40L exist, often associated with pathology and leading to truncated or nontrimerizable forms of CD40L (8). While CD40L is a ligand for CD40, the ligation of CD40L by CD40 initiates bidirectional signaling in both CD40 and CD40L expressing cells (10). The extracellular region of human CD40L is 99%, 88%, 86%, 82%, 75% and 75% aa identical to the extracellular regions of CD40L in rhesus monkey, bovine, porcine, canine, mouse and rat, respectively. CD40L binds to both CD40 and to integrin  alpha IIb beta 3 (CD41) (3, 11). In the cell membrane, it also associates with a unique splice variant of CD28 (CD28i) that may facilitate CD40L signal transduction (12). CD40L is expressed by monocytes, NK cells, mast cells, basophils, smooth muscle cells, endothelial cells, dendritic cells, activated and resting B cells, plus activated platelets and CD4+ T cells (13, 14). CD40L ligation of CD40 on dendritic cells (DC) initiates DC maturation and differentiation. CD40L signaling into naïve B cells promotes germinal center formation and isotope switching. With IL-21, CD40L generates IgA plus IgG3; with IL-4, CD40L generates IgG1 secretion (14, 15).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, Blocking, IHC, Neutralization.

Showing Publications 1 - 7 of 7.

Publications using MAB617	Applications	Species
S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)	Neutralization	Human
DE Buchholz, TS Carroll, A Kocabas, X Zhu, H Behesti, PL Faust, L Stalbow, Y Fang, ME Hatten Novel genetic features of human and mouse Purkinje cell differentiation defined by comparative transcriptomics Proc. Natl. Acad. Sci. U.S.A., 2020-06-16;117(26):15085-15095. 2020-06-16 [PMID: 32546527] (IHC, Human)	IHC	Human
Damir Bojadzic, Peter Buchwald CD40-targeting KGYY15 peptides do not efficiently block the CD40–CD40L interaction Diabetologia 2019-11-01 [PMID: 31501919]
D Bojadzic, J Chen, O Alcazar, P Buchwald Design, Synthesis, and Evaluation of Novel Immunomodulatory Small Molecules Targeting the CD40?CD154 Costimulatory Protein-Protein Interaction Molecules, 2018-05-11;23(5):. 2018-05-11 [PMID: 29751636] (Bioassay, Human)	Bioassay	Human
C Sun, SB Feng, ZW Cao, JJ Bei, Q Chen, WB Zhao, XJ Xu, Z Zhou, ZP Yu, HY Hu Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis Cell. Physiol. Biochem., 2017-04-27;41(6):2319-2332. 2017-04-27 [PMID: 28463834] (Neutralization, Human)	Neutralization	Human
Maniar A, Zhang X, Lin W, Gastman BR, Pauza CD, Strome SE, Chapoval AI Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood, 2010-06-02;116(10):1726-33. 2010-06-02 [PMID: 20519625] (Neutralization, Human)	Neutralization	Human
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-04-01;38(4):1012-23. 2008-04-01 [PMID: 18350544] (Blocking, Human)	Blocking	Human

Array MAB617

• CD40 Ligand/TNFSF5 Antibodies
• CD40 Ligand/TNFSF5 ELISA Kits
• CD40 Ligand/TNFSF5 Lysate
• CD40 Ligand/TNFSF5 Proteins
• CD40 Ligand/TNFSF5 Support Product
• CD40 Ligand/TNFSF5 ELISAs
• CD40 Ligand/TNFSF5 Proteins and Enzymes