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CD34 Antibody [Biotin]

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF3890
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3890
Carrier Free
AF3890
Carrier Free
AF3890-SP
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CD34 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant porcine CD34
Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn)
Accession # NP_999251
Specificity
Detects porcine CD34 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant rat CD34 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD34
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD34 Antibody [Biotin]

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20‑294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD34 Antibody (BAF3890). (Showing 1 - 1 of 1 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD34
Uniprot