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CD34 Antibody

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
Biotin
BAF3890
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Catalog# & Formulation Size Price
Carrier Free
AF3890-SP
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CD34 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant porcine CD34
Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn)
Accession # NP_999251
Specificity
Detects porcine CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant rat CD34 is observed, and less than 5% cross-reactivity with recombinant mouse CD34 and recombinant human CD34 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD34
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3890 in the following application:
Publications
Read Publication using AF3890.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD34 Antibody

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD34 Antibody (AF3890)(1)


Review for CD34 Antibody (AF3890) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Porcine.

Reviews using AF3890:
Filter by Applications
IHC-Fr
(1)
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Porcine
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD34 AF3890
Enlarge 		5
reviewed by:
Hanane Belhoul-Fakir
 IHC-Fr Porcine 12/03/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedArteries,porcine arteries
SpeciesPorcine
LotYST022009A

Comments

CommentsPig healthy arterial sections of 7µm were fixed in ice cold acetone for 7min. Once dry, blocking buffer 4% FCS/TBS was added to all tissues for 30min to minimize non-specific binding, followed by 10 min incubation with Bloxall, endogenous peroxidase blocking solution at room temperature. Tissues were washed in Tris buffered saline (TBS) for 5 min and incubated for 20min with normal horse serum (Vector laboratories # S-2012-50). Polyclonal goat anti-CD34 (R&D systems # AF3890) was diluted at 1:80 in 0.5% FCS/TBS and added to the arterial tissues for 1 hour incubation at room temperature. Following 5 min wash in TBS, tissues were incubated for 30min with ImmPRESS®HRP horse anti-goat reagent (Vector laboratories # MP-7405). To detect CD34 antigen, tissues were washed in TBS and incubated with VIP HRP substrate (Vector®VIP Substrate kit, Peroxidase (HRP) # SK-4600) for 1 min 30sec then placed immediately in MilliQ water to stop the reaction. Nuclei were counter-stained with methyl green (Sigma-Aldrich # M8884-25G). Control tissues were stained similarly in the absence of primary antibodies. Tissue sections were immediately dehydrated and mounted with ENTELLAN mounting medium (Proscitech, Cat#IA013). Images were captured on Nikon eclipse ME600 microscope and DS-Fi2 camera.

Product General Protocols

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Video Protocols

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FAQs for CD34 Antibody (AF3890). (Showing 1 - 1 of 1 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

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Recent Reviews

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5
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1
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Immunohistochemistry-Frozen CD34 AF3890
Enlarge
 Hanane Belhoul-Fakir
12/03/2020
Application: IHC-Fr
Species: Porcine
read this review

Bioinformatics

Gene Symbol CD34
Uniprot