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CD300c Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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CD300c Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD300c/LMIR2
Met29-Arg183
Accession # Q08708
Specificity
Detects human CD300c in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) LMIR1 is observed and less than 1% cross-reactivity with rhLMIR3, rhLMIR4, rhLMIR5, and rhLMIR6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD300C
Purity Statement
Antigen Affinity-purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD300c Antibody [Biotin]

  • CD300c antigenCMRF35 antigen
  • CD300c molecule
  • CD300c
  • CLM6
  • CLM-6
  • CMRF35 leukocyte immunoglobulin-like receptor
  • CMRF35
  • CMRF-35
  • CMRF35A leukocyte immunoglobulin-like receptor
  • CMRF-35A
  • CMRF35ACMRF35A1
  • CMRF35CMRF35-A1
  • CMRF35-like molecule 6
  • IGSF16
  • IgSF16
  • IGSF16CD300 antigen-like family member C
  • Immunoglobulin superfamily member 16
  • LIR

Background

Leukocyte mono-Ig-like receptor 2 (LMIR2; also CMRF-35, CMRF35-A antigen, and CD300c antigen) is a 23 kDa (predicted) type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of molecules within the immunoglobulin (Ig) superfamily (1‑4). Human LMIR2 is synthesized as a 224 amino acid (aa) precursor that has a 20 aa signal sequence, a 163 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 20 aa cytoplasmic tail. The ECD contains an Ig-like V-type domain (aa’s 22‑128) and two N-linked glycosylation sites (aa’s 90 and 99). Downstream of the Ig V-domain, the membrane proximal region of LMIR2 (aa 128‑183) contains a high proportion of proline (18%), serine (20%) and threonine (13%) residues (1). The transmembrane segment contains a charged glutamic acid that contributes to cell activation (1‑3). Human LMIR2 shares 52% aa sequence identity with the mouse LMIR2, which is also known as CLM4. Human LMIR2 is 90% identical to human LMIR1 within the Ig-like domain. LMIR2 is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes (1, 3). Mouse LMIR2 has the characteristics of an activatory molecule capable of inducing cellular activation and effector function in most cells and macrophages (3). The ligand for LMIR2 is presently unknown.

  1. Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
  2. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
  3. Clark, G.J. et al. (2002) J. Biol. Regul. Homeost. Agents 16:233.
  4. Daish, A. et al. (1993) Immunology 79:55.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

FAQs for CD300c Antibody (BAF3256). (Showing 1 - 1 of 1 FAQ).

  1. Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?
    • A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:https://www.uniprot.org/uniprot/Q08708#sequencesWe can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.
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Bioinformatics

Gene Symbol CD300C
Uniprot