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CD3 gamma Antibody (5B7B2) - BSA Free

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Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mAb against HEK293 (1) and CD3G (AA: extra 23-116)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis of Raji cells using CD3G mouse mAb (green) and negative control (red).
Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mAb against human CD3G (AA: extra 23-116) recombinant protein. (Expected MW is 36.6 kDa)
Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mouse mAb against Jurkat (1) cell lysate.
ELISA: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clone
5B7B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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CD3 gamma Antibody (5B7B2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD3 gamma (AA: extra 23-116) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD3G
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD3 gamma Antibody (5B7B2) - BSA Free

  • CD3 gamma
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3g
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ79544
  • FLJ94613
  • IMD17
  • MGC138597
  • T3G
  • T-cell antigen receptor complex, gamma subunit of T3
  • T-cell receptor T3 gamma chain
  • T-cell surface glycoprotein CD3 gamma chain

Background

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD3G