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CD19 Antibody (771404)

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Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD19 at approximately 60-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog ...read more
CD19 was detected in perfusion fixed frozen sections of rat spleen using Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clone
771404
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7489-SP
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CD19 Antibody (771404) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant rat CD19
Met1-Gly287
Accession # NP_001013255
Specificity
Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CD19 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB7489.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD19 Antibody (771404)

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

CD19 (also surface antigen B4 and Leu12) is a 95‑110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19‑547). Based on mouse, it contains a 269 aa extracellular region (aa 19‑287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20‑113 and 171‑271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19‑287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD19
Uniprot