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CD11c Antibody (CL1831)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues. Corresponding ITGAX RNA-seq data are presented for the same tissues.
Western Blot: CD11c Antibody (CL1831) [NBP2-34491] - Lane 1: Marker [kDa]. Lane 2: Human tonsil.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - CD11c Positive cells in mouse liver tissue. Primary antibody dilution: 1:100. Citrate buffer antigen retrieval. Overnight incubation RT. Image from ...read more
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human tonsil shows strong membranous positivity in a subset of germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human small intestine shows moderate positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human fallopian tube shows moderate membranous positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human kidney shows no positivity as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
CL1831
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CD11c Antibody (CL1831) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRV
Epitope
LYIDKRSKNLLGSRD
Marker
Dendritic Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAX
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CD11c Recombinant Protein Antigen (NBP2-34491PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-34491 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CD11c Antibody (CL1831)

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (NBP2-34491) (0)

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Review for CD11c Antibody (NBP2-34491) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-34491:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Mozhdeh Sojoodi
IHC-P Mouse 02/07/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver tissue,Liver
SpeciesMouse
LotCL1831

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
1:100 dilution
Citrate buffer antigen retrieval
Overnight incubation RT

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD11c Antibody (NBP2-34491) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD11c Antibody (NBP2-34491)

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Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Recent Reviews

4
5
0
4
1
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2
0
1
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Mozhdeh Sojoodi
02/07/2022
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol ITGAX
Uniprot