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CD11c Antibody (435421) [Unconjugated]

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Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11c at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
CD11c was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB6950) at 5 µg/ml for 1 hour at room temperature followed by incubation with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
435421
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6950-SP
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CD11c Antibody (435421) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD11c
Phe20-Pro1116
Accession # Q9QXH4
Marker
Dendritic Cell Marker
Specificity
Detects mouse CD11c in direct ELISAs and Western blots. In direct ELISAs, 50%‑100% cross‑reactivity with recombinant human (rh) Integrin  alpha X beta 2 and recombinant mouse (rm) Integrin alpha L is observed, 10%‑25% cross‑reactivity with rhIntegrin CD11c, rhIntegrin alpha D beta 2, and rhIntegrin alpha L beta 2 is observed and no cross-reactivity with rhIntegrin alpha M or rhIntegrin beta 2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ITGAX
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB6950 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD11c Antibody (435421) [Unconjugated]

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat CD11c, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen) while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (MAB6950)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ITGAX
Uniprot