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CD11b/c Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-40766AF350
Alexa Fluor 405
NB110-40766AF405
Alexa Fluor 488
NB110-40766AF488
Alexa Fluor 532
NB110-40766AF532
Alexa Fluor 594
NB110-40766AF594
Alexa Fluor 647
NB110-40766AF647
Alexa Fluor 700
NB110-40766AF700
Alexa Fluor 750
NB110-40766AF750
Allophycocyanin
NB110-40766APC
Allophycocyanin/Cy7
NB110-40766APCCY7
Biotin
NB110-40766B
CoraFluor 1
NB110-40766CL1
DyLight 350
NB110-40766UV
DyLight 405
NB110-40766V
DyLight 488
NB110-40766G
DyLight 550
NB110-40766R
DyLight 594
NB110-40766DL594
DyLight 650
NB110-40766C
DyLight 680
NB110-40766FR
DyLight 755
NB110-40766IR
FITC
NB110-40766F
Janelia Fluor 525
NB110-40766JF525
Janelia Fluor 549
NB110-40766JF549
Janelia Fluor 646
NB110-40766JF646
Janelia Fluor 669
NB110-40766JF669
PE
NB110-40766PE
PE/Atto594
NB110-40766PEATT594
PE/Cy5.5
NB110-40766PECY55
PE/Cy7
NB110-40766PECY7
PerCP
NB110-40766PCP
Unconjugated
NB110-40766
mFluor Violet 450 SE
NB110-40766MFV450
mFluor Violet 500 SE
NB110-40766MFV500
mFluor Violet 610 SE
NB110-40766MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB110-40766
BSA Free
NB110-40766
BSA Free
NB110-40766SS
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CD11b/c Antibody [HRP] Summary

Immunogen
A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein. [Swiss-Prot: P05555]
Localization
Monocytes and granulocytes.
Marker
Microglia Marker, Macrophage Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25150592)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for CD11b/c Antibody [HRP]

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • CD11b
  • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
  • Cell surface glycoprotein MAC-1 subunit alpha
  • CR-3 alpha chain
  • CR3AMGC117044
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • Leukocyte adhesion receptor MO1
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD11b/c Antibody (NB110-40766H). (Showing 1 - 3 of 3 FAQ).

  1. Besides storing the antibody in the dark, do I need to follow the whole experimental procedure (for IHC) in the dark?
    • We recommend that all of our directly conjugated antibodies be handled in a light sensitive manner to prevent photobleaching or reduced signal by light degradation. HRP is a more robust dye compared to fluorescent dyes, but should still be kept and stored in a dark or opaque vial. It is not necessary to run all steps of your IHC in the dark, but if you can cover or keep your slides out of direct light during primary antibody incubation and after, that is recommended.
  2. Do you think that the results may be effected by the light of the microscope during analysis? How might you deal with this problem?
    • The light from your microscope should not affect the HRP as you should have already added your colorimetric reagents (such as DAB or ABC). Once your color change has occurred, the visible color is stable.
  3. Being HRP conjugated, what blocking reagent should be used for IHC?
    • You will not need to use any specific blocking buffer for IHC with this directly conjugated antibody. The antibody simply makes the IHC process easier by eliminating the need for a secondary antibody. You can use either normal goat serum or BSA as a blocking reagent (since you do not have to match the serum to the host species).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITGAM