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CD117/c-kit Antibody (CL1657)

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Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Western Blot: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human stomach shows strong positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human cerebellum shows moderate positivity in neuropil of the molecular layer.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human colon shows strong immunoreactivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human pancreas shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (CL1657) [NBP2-52975] - Staining of human small intestine shows strong immunoreactivity in a subset of lymphoid cells (macrophages).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CL1657
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CD117/c-kit Antibody (CL1657) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQ
Epitope
ILDETNENKQNEWIT
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CD117/c-kit Recombinant Protein Antigen (NBP2-52975PEP)
Publications
Read Publications using
NBP2-52975 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CD117/c-kit Antibody (CL1657)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD117/c-kit
Antibody (CL1657)
NBP2-52975
CD117/c-kit Antibody (CL1657)
Species: Hu
Applications: WB, IHC

Publications for CD117/c-kit Antibody (NBP2-52975)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, IHC-P.


Filter By Application
IF/IHC
(1)
IHC-P
(1)
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Human
(2)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-52975 Applications Species
MOHEBAT H. GOUDA M.D. M, NEHAL S. ZAFER M.D. T, ELBAZ M.D. M Chromophobe Renal Cell Carcinoma, Oncocytoma and Clear Cell Carcinoma: A Compartive Immunohistochemical Study The Medical Journal of Cairo University 2020-03-01 (IF/IHC, Human) IF/IHC Human
Gideon HP, Hughes TK, Tzouanas CN et al. Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control Immunity 2022-04-26 [PMID: 35483355] (IHC-P, Human) IHC-P Human

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Secondary Antibodies

 

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT