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CD117/c-kit Antibody (47233) [Janelia Fluor® 646]

 
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, B/N, CyTOF-ready, ELISA, ELISA(Cap), ELISA(Det)
Clone
47233
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB332U-100UG
Alexa Fluor 405
FAB332V-100UG
Alexa Fluor 488
FAB332G-100UG
Alexa Fluor 532
FAB332X
Alexa Fluor 594
FAB332T-100UG
Alexa Fluor 647
FAB332R-100UG
Alexa Fluor 700
FAB332N-100UG
Alexa Fluor 750
FAB332S-100UG
Allophycocyanin
FAB332A-100
Allophycocyanin
FAB332A-025
Allophycocyanin/Cy7
FAB332APCCY7
Biotin
FAB332B
CoraFluor 1
FAB332CL1
DyLight 350
FAB332D
DyLight 405
FAB332E
DyLight 488
FAB332K
DyLight 550
FAB332L
DyLight 594
FAB332M
DyLight 650
FAB332W
DyLight 680
FAB332Y
DyLight 755
FAB332Z
FITC
FAB332F
HRP
FAB332H
Janelia Fluor 525
FAB332JF525
Janelia Fluor 549
FAB332I
Janelia Fluor 585
FAB332JF585
Janelia Fluor 635
FAB332JF635
Janelia Fluor 669
FAB332JF669
PE/Atto594
FAB332PEATT594
PE/Cy5.5
FAB332PECY55
PE/Cy7
FAB332PECY7
Phycoerythrin
FAB332P-025
Phycoerythrin
FAB332P-100
Unconjugated
MAB332-100
mFluor Violet 450 SE
FAB332MFV450
mFluor Violet 500 SE
FAB332MFV500
mFluor Violet 610 SE
FAB332MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB332-100
Carrier Free
MAB332-SP
Carrier Free
MAB332-100
Carrier Free
MAB332-500
Carrier Free
FAB332A-100
Carrier Free
FAB332A-025
Carrier Free
FAB332P-100
Carrier Free
FAB332P-025
Carrier Free
FAB332S-100UG
Carrier Free
FAB332R-100UG
Carrier Free
FAB332N-100UG
Carrier Free
FAB332G-100UG
Carrier Free
FAB332V-100UG
Carrier Free
FAB332U-100UG
Carrier Free
FAB332T-100UG
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CD117/c-kit Antibody (47233) [Janelia Fluor® 646] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit
Gln26-Thr520
Accession # P10721
Specificity
Detects human CD117/c-kit in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross-reactivity with recombinant mouse CD117 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Flow Cytometry
  • Neutralization
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from ascites

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD117/c-kit Antibody (47233) [Janelia Fluor® 646]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD117/c-kit
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FAB332J
CD117/c-kit Antibody (47233) [Janelia Fluor® 646]
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Secondary Antibodies

 

Isotype Controls

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Blogs on CD117/c-kit.

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT