Home » CCL7/MCP-3/MARC » CCL7/MCP-3/MARC Antibodies » CCL7/MCP-3/MARC Antibody (36320) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CCL7/MCP-3/MARC Antibody (36320) [Unconjugated]

 (5 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Human CCL7/MCP‑3 (Catalog #282-P3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-HumanCCL7/MCP‑3 Monoclonal Antibody (Catalog # MAB282). The ND50 is typically1.5-4.5 µg/mL.
Recombinant Human CCL7/MCP‑3 (Catalog #282-P3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, CyTOF-ready, ELISA(Cap), Flow
Clone
36320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC282U-100UG
Alexa Fluor 405
IC282V-100UG
Alexa Fluor 488
IC282G-100UG
Alexa Fluor 532
FAB282X
Alexa Fluor 594
IC282T-100UG
Alexa Fluor 647
IC282R-100UG
Alexa Fluor 700
IC282N-100UG
Alexa Fluor 750
IC282S-100UG
Allophycocyanin
FAB282A
Biotin
FAB282B
CoraFluor 1
FAB282CL1
DyLight 350
FAB282D
DyLight 405
FAB282E
DyLight 488
FAB282K
DyLight 550
FAB282L
DyLight 594
FAB282M
DyLight 650
FAB282W
DyLight 680
FAB282Y
DyLight 755
FAB282Z
FITC
FAB282F
HRP
FAB282H
Janelia Fluor 525
FAB282JF525
Janelia Fluor 549
FAB282I
Janelia Fluor 646
FAB282J
Janelia Fluor 669
FAB282JF669
PE
FAB282P
PerCP
FAB282C
mFluor Violet 450 SE
FAB282MFV450
mFluor Violet 500 SE
FAB282MFV500
mFluor Violet 610 SE
FAB282MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB282-SP
Carrier Free
MAB282-500
Carrier Free
IC282V-100UG
Carrier Free
IC282U-100UG
Carrier Free
IC282T-100UG
Carrier Free
IC282S-100UG
Carrier Free
IC282R-100UG
Carrier Free
IC282N-100UG
Carrier Free
IC282G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CCL7/MCP-3/MARC Antibody (36320) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL7/MCP-3/MARC
Gln34-109
Accession # Q7Z7Q8
Specificity
Detects human CCL7/MCP-3/MARC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL2/MCP-1, rhCCL8/MCP‑2, or rhCCL13/MCP‑4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL7
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 1.5-4.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human CCL7/MCP-3/MARC Biotinylated Antibody (Catalog number BAF282)
Standard: Recombinant Human CCL7/MCP-3/MARC (Catalog number 282-P3)
Publications
Read Publications using
MAB282 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL7/MCP-3/MARC Antibody (36320) [Unconjugated]

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(256 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CCL8/MCP-2 [Biotin]
DY790
N/A CCL8/MCP-2 [Biotin]
(11 Publications)
Species: Mu
Applications: ELISA
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CCL13/MCP-4
[Biotin]
DY327
N/A CCL13/MCP-4 [Biotin]
(12 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
CCR2 Antibody
(48607) [Unconjugated
MAB150
Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]
Flow Cytometry CCR2 Antibody (48607) [Unconjugated]
(45 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(155 Publications)
Species: Hu
Applications: ELISA
CD46 Antibody
(344519) [Unconjugate
MAB2005
N/A CD46 Antibody (344519) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, WB
CapG Antibody
NBP1-90214
Immunocytochemistry/ Immunofluorescence CapG Antibody
Western Blot CapG Antibody
Immunohistochemistry-Paraffin CapG Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(183 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(665 Publications)
Species: Mu
Applications: ELISA
CCR3 Antibody
(61828)
MAB155
Immunohistochemistry CCR3 Antibody (61828)
Neutralization CCR3 Antibody (61828)
(20 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CCR1 Antibody
(53504) [Unconjugated
MAB145
Flow Cytometry CCR1 Antibody (53504) [Unconjugated]
(1 Review)
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
CXCR1/IL-8RA
Antibody (42705) [Unc ...
MAB330
Flow Cytometry CXCR1/IL-8RA Antibody (42705) [Unconjugated]
Neutralization CXCR1/IL-8RA Antibody (42705) [Unconjugated]
(50 Publications)
Species: Hu
Applications: CyTOF-reported, Flow, IHC, Neut
CXCL9/MIG Antibody
[Unconjugated]
AF-492-NA
Neutralization CXCL9/MIG Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]
Western Blot CXCL9/MIG Antibody [Unconjugated]
(31 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA

Publications for CCL7/MCP-3/MARC Antibody (MAB282)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
ICC
(1)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB282 Applications Species
F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (ICC, Human) ICC Human
J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Redox Biol, 2019-02-06;22(0):101116. 2019-02-06 [PMID: 30769285] (Neutralization, Human) Neutralization Human
Victor Laurent, Adrien Guérard, Catherine Mazerolles, Sophie Le Gonidec, Aurélie Toulet, Laurence Nieto et al. Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity Nature Communications 1/12/2016 [PMID: 26756352]
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010-07-15;127(2):332-44. 2010-07-15 [PMID: 19937793] (IHC-P, Neutralization, Human) IHC-P, Neutralization Human
Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2005-08-17;65(3):294-300. 2005-08-17 [PMID: 16107514] (IHC-Fr, Human) IHC-Fr Human

Reviews for CCL7/MCP-3/MARC Antibody (MAB282) (0)

There are no reviews for CCL7/MCP-3/MARC Antibody (MAB282). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL7/MCP-3/MARC Antibody (MAB282) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CCL7/MCP-3/MARC Products

Blogs on CCL7/MCP-3/MARC

There are no specific blogs for CCL7/MCP-3/MARC, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCL7/MCP-3/MARC Antibody (36320) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL7
Uniprot