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CCL20/MIP-3 alpha Overexpression Lysate

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Western Blot: Macrophage Inflammatory Protein 3 alpha Overexpression Lysate (Adult Normal) [NBL1-08850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Macrophage Inflammatory Protein 3 alpha.
Western Blot: Macrophage Inflammatory Protein 3 alpha Overexpression Lysate (Adult Normal) [NBL1-08850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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CCL20/MIP-3 alpha Overexpression Lysate Summary

Description

CCL20/MIP-3 alpha Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204691

Accession#: NM_004591

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CCL20

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
10.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CCL20/MIP-3 alpha Overexpression Lysate

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibitsproliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of themucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processedforms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coliATCC 25922 and S.aureus ATCC 29213

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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CCL20/MIP-3 alpha
Overexpression Lysate
NBL1-08850
CCL20/MIP-3 alpha Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol CCL20