CCL20/MIP-3 alpha ELISA Development Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Macrophage inflammatory protein (MIP-3a)/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3a plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3a is the most potent chemokine inducing the selective migration of in vitro-generated CD34+ hematopoietic progenitor cell-derived LC precursors and skin LCs, in accordance with the restricted MIP-3a receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3a is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked by an antibody against MIP-3a. (c) In vivo, MIP-3a is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3a up regulation is associated with an increase in LC turnover. (d) The secretion of MIP-3a is strongly up regulated by cells of epithelial origin after inflammatory stimuli (interleukin 1b plus TNFa) or T cell signals (Homey B. et al, 2000, J.Immunol. 164:6621-32). Monoclonal antibodies anti-MIP3a were obtained after mice immunization with MIP-3a-transfected eukaryotic cells. These clones were used to set up a human MIP-3a specific Elisa, which also detect macaque MIP3a. No cross-reactivity with MIP-1a, MIP-1b, MIP-1d, MIP-3b, eotaxin, 6Ckine, Rantes, MCP-1, TARC, MDC, TECK, SDF-1 and IP-10 was detected (Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 1 confirmed species: Rat.

Showing Publications 1 - 4 of 4.

Publications using DDXK-E-MIP-3-alpha	Applications	Species
Li Q et al. Glycerol monolaurate prevents mucosal SIV transmission. Nature 2009-04-23 [PMID: 19262509]
Doi T, Sakoda T, Akagami T et al. Aldosterone induces interleukin-18 through endothelin-1, angiotensin II, Rho/Rho-kinase, and PPARs in cardiomyocytes. Am J Physiol Heart Circ Physiol. 2008-09-01 [PMID: 18660453] (Rat) Details: Primary cultures of rat neonatal rat cardiomyoctyes treated with various stimulators or drugs: Figs 7A-7B, Supplemental Figs. S3A-S3D.		Rat
Dieu-Nosjean MC et al. Macrophage inflammatory protein 3alpha is expressed at inflamed epithelial surfaces and is the most potent chemokine known in attracting Langerhans cell precursors. J Exp Med 2000-09-04 [PMID: 10974036]
Homey B, Dieu-Nosjean MC, Wiesenborn A et al. Up-regulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis. J Immunol 2000-06-15 [PMID: 10843722]

Array DDXK-E-MIP-3-alpha

• CCL20/MIP-3 alpha Antibodies
• CCL20/MIP-3 alpha Antibody Pair
• CCL20/MIP-3 alpha ELISA Kits
• CCL20/MIP-3 alpha Kit
• CCL20/MIP-3 alpha Lysate
• CCL20/MIP-3 alpha Proteins
• CCL20/MIP-3 alpha ELISAs
• CCL20/MIP-3 alpha Proteins and Enzymes