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CCL20/MIP-3 alpha Antibody [Biotin]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF540
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF540
Carrier Free
AF540
Carrier Free
AF540-SP
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CCL20/MIP-3 alpha Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant rat CCL20/MIP‑3 alpha
Ala26-Met96
Accession # P97884
Specificity
Detects rat CCL20/MIP‑3 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) MIP-3 alpha , recombinant mouse (rm) MIP-3 alpha , rhMIP-3 beta and rmMIP-3 beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL20
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Rat CCL20/MIP-3 alpha Antibody (Catalog # MAB540)
ELISA Detection: Rat CCL20/MIP-3 alpha Biotinylated Antibody (Catalog # BAF540)
Standard: Recombinant Rat CCL20/MIP-3 alpha (Catalog # 540-RM)
Readout System
Publications
Read Publication using BAF540.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody [Biotin]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

CCL20, also known as MIP-3 alpha , LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity). Rat MIP-3 alpha shares approximately 70 and 61% aa sequence homology with mouse and human CCL20, respectively.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL‑10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha
Antibody [Biotin]
BAF540
CCL20/MIP-3 alpha Antibody [Biotin]
Species: Rt
Applications: WB, ELISA(Det)

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Bioinformatics

Gene Symbol CCL20
Uniprot