Home » CCL20/MIP-3 alpha » CCL20/MIP-3 alpha Antibodies » CCL20/MIP-3 alpha Antibody (114908) [Unconjugated]
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CCL20/MIP-3 alpha Antibody (114908) [Unconjugated]

 (15 Publications)
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Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601). The ND50 is typically 3-15 µg/mL.
Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
114908
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7601-SP
Carrier Free
MAB7601-500
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CCL20/MIP-3 alpha Antibody (114908) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093
Specificity
Detects mouse CCL20/MIP-3 alpha in ELISAs. Does not cross-react with recombinant human (rh) CCL19, rhCCL20, recombinant mouse (rm) CCL3, 4, 9, 19, rmCXCL2, or recombinant rat CCL20.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CCL20
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 3-15 ug/mL
Application Notes
ELISA Detection: Mouse CCL20/MIP-3alpha Biotinylated Antibody (Catalog number BAF760)
Standard: Recombinant Mouse CCL20/MIP-3alpha (Catalog number 760-M3)
Publications
Read Publications using
MAB7601 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody (114908) [Unconjugated]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

MIP-3 alpha , also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha , respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha Antibody (114908) [Unconjugated]
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Publications for CCL20/MIP-3 alpha Antibody (MAB7601)(15)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: Bioassay, ELISA Development, ELISA Development (Capture), In Vivo, Neutralization.


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Bioassay
(1)
ELISA Development
(1)
ELISA Development (Capture)
(2)
In Vivo
(4)
Neutralization
(6)
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Mouse
(13)
Rat
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB7601 Applications Species
Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF; IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils Cell reports 2023-06-08 [PMID: 37300834] (In Vivo, Mouse) In Vivo Mouse
T Hirz, S Mei, H Sarkar, Y Kfoury, S Wu, BM Verhoeven, AO Subtelny, DV Zlatev, MW Wszolek, K Salari, E Murray, F Chen, EZ Macosko, CL Wu, DT Scadden, DM Dahl, N Baryawno, PJ Saylor, PV Kharchenko, DB Sykes Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses Nature Communications, 2023-02-07;14(1):663. 2023-02-07 [PMID: 36750562] (In Vivo, Mouse) In Vivo Mouse
M Bruchard, M Geindreau, A Perrichet, C Truntzer, E Ballot, R Boidot, C Racoeur, E Barsac, F Chalmin, C Hibos, T Baranek, C Paget, B Ryffel, C Rébé, C Paul, F Végran, F Ghiringhel Recruitment and activation of type 3 innate lymphoid cells promote antitumor immune responses Nature Immunology, 2022-01-31;23(2):262-274. 2022-01-31 [PMID: 35102345] (In Vivo, Mouse) In Vivo Mouse
Mingcan Yu, Subhashis Pal, Cameron W. Paterson, Jau-Yi Li, Abdul Malik Tyagi, Jonathan Adams et al. Ovariectomy induces bone loss via microbial-dependent trafficking of intestinal TNF+ T cells and Th17 cells Journal of Clinical Investigation 2/15/2021 [PMID: 33586672]
JW Shin, MA Kwon, J Hwang, SJ Lee, JH Lee, HJ Kim, KB Lee, SJ Lee, EM Jeong, JH Chung, IG Kim Keratinocyte transglutaminase 2 promotes CCR6+ &amp;gamma&amp;deltaT-cell recruitment by upregulating CCL20 in psoriatic inflammation Cell Death Dis, 2020-04-30;11(4):301. 2020-04-30 [PMID: 32355189] (Bioassay, Mouse) Bioassay Mouse
M Yu, A Malik Tyag, JY Li, J Adams, TL Denning, MN Weitzmann, RM Jones, R Pacifici PTH induces bone loss via microbial-dependent expansion of intestinal TNF+ T cells and Th17 cells Nat Commun, 2020-01-24;11(1):468. 2020-01-24 [PMID: 31980603] (In Vivo, Rat) In Vivo Rat
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Ulf Panzer Autoimmune Renal Disease Is Exacerbated by S1P-Receptor-1-Dependent Intestinal Th17 Cell Migration to the Kidney Immunity, 2016-11-15;45(5):1078-1092. 2016-11-15 [PMID: 27851911] (Neutralization, Mouse) Neutralization Mouse
Boyle S, Faulkner J, McColl S, Kochetkova M The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages. Mol Cancer, 2015-06-06;14(0):115. 2015-06-06 [PMID: 26047945] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis. J Invest Dermatol, 2012-08-16;133(1):164-71. 2012-08-16 [PMID: 22895364] (Neutralization, Mouse) Neutralization Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol CCL20
Uniprot