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CCL20/MIP-3 alpha Antibody (114906) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of mouse small intestine. PVDF membrane was probed with 2 µg/mL of Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL20 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse small intestine. PVDF membrane was probed with 2 µg/mL of Mouse CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
Conditional macrophage ablation disrupted Treg-cell recruitment and inhibited the growth of CRC in mice.(A, B) Flow cytometric analysis of TAMs derived from grafted CRC in CD11b-DTR mice injected with DTmu or DT. (C) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
114906
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB760U
Alexa Fluor 405
FAB760V
Alexa Fluor 488
FAB760G
Alexa Fluor 532
FAB760X
Alexa Fluor 594
FAB760T
Alexa Fluor 647
FAB760R
Alexa Fluor 750
FAB760S
Biotin
FAB760B
CoraFluor 1
FAB760CL1
DyLight 350
FAB760D
DyLight 405
FAB760E
DyLight 488
FAB760K
DyLight 550
FAB760L
DyLight 594
FAB760M
DyLight 650
FAB760W
DyLight 680
FAB760Y
DyLight 755
FAB760Z
FITC
FAB760F
HRP
FAB760H
Janelia Fluor 525
FAB760JF525
Janelia Fluor 549
FAB760I
Janelia Fluor 635
FAB760JF635
Janelia Fluor 646
FAB760J
Janelia Fluor 669
FAB760JF669
PE
FAB760P
PerCP
FAB760C
mFluor Violet 450 SE
FAB760MFV450
mFluor Violet 500 SE
FAB760MFV500
mFluor Violet 610 SE
FAB760MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB760-SP
Carrier Free
MAB760-500
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CCL20/MIP-3 alpha Antibody (114906) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala28-Met97
Accession # O89093
Specificity
Detects mouse CCL20/MIP‑3 alpha in direct ELISAs and Western blots. In direct ELISAs, 12% cross-reactivity with recombinant rat (rr) CCL20, 6% cross-reactivity with recombinant mouse (rm) CCL12 and recombinant human (rh) CCL25, and 3% cross‑reactivity with with rmCCL9/10/MIP-1 gamma is observed. No cross-reactivity with recombinant cotton rat CCL3, 4, 5, rhCCL1, 2, 3, 4, 5, 7, 8, 11 ,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, rmCCL1, 2, 3, 4, 5, 6, 7, 8, 11, 17, 19, 21, 22, 24, 25, 27, or 28 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CCL20
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB760 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody (114906) [Unconjugated]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

CCL20, also known as MIP-3 alpha , LARC, and Exodus, is a beta chemokine. Mouse CCL20 cDNA encodes a 97 amino acid precursor protein with a 27 aa putative signal peptide that is predicted to be cleaved to form the 70 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity). Mouse CCL20 shares approximately 71 and 63% amino acid sequence homology with rat and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 is a functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha
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MAB760
CCL20/MIP-3 alpha Antibody (114906) [Unconjugated]
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Publications for CCL20/MIP-3 alpha Antibody (MAB760)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Neutralization.


Filter By Application
IHC
(1)
Neutralization
(5)
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Mouse
(6)
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Showing Publications 1 - 7 of 7.
Publications using MAB760 Applications Species
L Lu, Y Wang, L Zhou, Y Li, X Zhang, X Hu, S Shi, W He V&gamma4 T cell-derived IL-17A is essential for amplification of inflammatory cascades in ischemic brain tissue after stroke International immunopharmacology, 2021-05-24;96(0):107678. 2021-05-24 [PMID: 34162129] (Neutralization, Mouse) Neutralization Mouse
M Das, X Tang, JY Han, K Mayilsamy, E Foran, MR Biswal, R Tzekov, SS Mohapatra, S Mohapatra CCL20-CCR6 axis modulated traumatic brain injury-induced visual pathologies J Neuroinflammation, 2019-05-31;16(1):115. 2019-05-31 [PMID: 31151410] (Neutralization, Mouse) Neutralization Mouse
Y Kawa, T Nagano, A Yoshizaki, R Dokuni, M Katsurada, T Terashita, Y Yasuda, K Umezawa, M Yamamoto, H Kamiryo, K Kobayashi, Y Nishimura Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells PLoS ONE, 2018-09-07;13(9):e0203211. 2018-09-07 [PMID: 30192865] (Neutralization, Mouse) Neutralization Mouse
James Sligh, Jaroslav Janda, Jana Jandova Mutations in BALB mitochondrial DNA induce CCL20 up-regulation promoting tumorigenic phenotypes Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2014-11-01 [PMID: 25177208]
Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE, 2014-04-03;9(4):e93057. 2014-04-03 [PMID: 24699535] (Neutralization, Mouse) Neutralization Mouse
Kashiwagi , Satoshi, Yuan , Jianping, Forbes , Benjamin, Hibert , Mathew L, Lee , Eugene L, Whicher , Laura, Goudie , Calum, Yang , Yuan, Chen , Tao, Edelblute , Beth, Collette , Brian, Edington , Laurel, Trussler , James, Nezivar , Jean, Leblanc , Pierre, Bronson , Roderick, Tsukada , Kosuke, Suematsu , Makoto, Dover , Jeffrey, Brauns , Timothy, Gelfand , Jeffrey, Poznansky , Mark C Near-infrared laser adjuvant for influenza vaccine. PLoS ONE, 2013-12-11;8(12):e82899. 2013-12-11 [PMID: 24349390] (IHC, Mouse) IHC Mouse
Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. J. Exp. Med., 2011-03-07;208(3):491-503. 2011-03-07 [PMID: 21383056] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol CCL20
Uniprot