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CCL2/JE/MCP-1 Antibody [Biotin]

 (21 Publications)
 (1 Review)
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Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma -overexpressing microparticles (black bars) or no microparticles (white bars) for 4 hours before activation with LPS or PAM3CSK4 for 24 hours. Supernatants were collected and pro-inflammatory cytokines IL-8 (top), MCP-1 (bottom) were measured by ELISA. Dotted line represents baseline cytokine production from unactivated cells with no microparticle exposure. Mean values with standard errors represent one of at least 3 experiments. Two-way ANOVA with Tukey's multiple comparison post test was performed to determine statistical significance. * indicates (p<0.05). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25426628), licensed under a CC-BY license. Not internally tested by R&D Systems.
Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma ...read more
Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-279-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-279-NA
Carrier Free
AF-279-NA
Carrier Free
AF-279-SP
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CCL2/JE/MCP-1 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL2/JE/MCP‑1 (R&D Systems, Catalog # 279-MC)
Gln24-Thr99
Accession # P13500
Specificity
Detects human CCL2/JE/MCP‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MCP‑2, rhMCP-3, rhMCP-4, recombinant mouse (rm) CCL2/JE/MCP‑1, and rmMARC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL2/JE/MCP-1 Antibody (Catalog # MAB679)
ELISA Detection: Human CCL2/JE/MCP-1 Biotinylated Antibody (Catalog # BAF279)
Standard: Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC)
Readout System
Reviewed Applications
Read 1 Review rated 5
using
BAF279 in the following applications:
Publications
Read Publications using
BAF279 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody [Biotin]

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the  beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported.

Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha , RANTES and MIP-1 beta . A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL2/JE/MCP-1 Antibody (BAF279)(21)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 8 applications: Antibody Array Development, ELISA, ELISA Detection, ELISA Development, ELISA Development (Detection), ICC, Luminex Development, Western Blot.


Filter By Application
Antibody Array Development
(1)
ELISA
(1)
ELISA Detection
(1)
ELISA Development
(7)
ELISA Development (Detection)
(2)
ICC
(1)
Luminex Development
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(17)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using BAF279 Applications Species
Hong, J;Son, M;Sin, J;Kim, H;Chung, DK; Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation Nutrients 2023-11-09 [PMID: 38004123] (ELISA Detection, Human) ELISA Detection Human
Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens Frontiers in Immunology 2020-08-25 [PMID: 32983121]
S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case Mediators Inflamm., 2020-07-06;2020(0):6087109. 2020-07-06 [PMID: 32694927] (ELISA, Human) ELISA Human
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 2020-08-01 [PMID: 32645325]
Sara Abouelasrar Salama, Mieke Gouwy, Alexandra De Zutter, Noëmie Pörtner, Lotte Vanbrabant, Nele Berghmans et al. Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1 beta and Interferon-gamma in Liver Bystander Cells Journal of Interferon & Cytokine Research 2020-04-01 [PMID: 32031878]
ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells Exp. Eye Res., 2018-02-16;170(0):29-39. 2018-02-16 [PMID: 29454857] (Western Blot, Human) Western Blot Human
RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury EBioMedicine, 2017-04-20;0(0):. 2017-04-20 [PMID: 28434946] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016-07-25;6(0):29909. 2016-07-25 [PMID: 27453287] (Human) Human
Sahler J, Woeller C, Phipps R Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes. PLoS ONE, 2014-11-26;9(11):e113189. 2014-11-26 [PMID: 25426628] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Xia Xi, David H. McMillan, Geniece M. Lehmann, Patricia J. Sime, Richard T. Libby, Krystel R. Huxlin et al. Ocular Fibroblast Diversity: Implications for Inflammation and Ocular Wound Healing Investigative Opthalmology & Visual Science 2011-07-01 [PMID: 21571679]
Show All 21 Publications.

Review for CCL2/JE/MCP-1 Antibody (BAF279) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using BAF279:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 ELISA Human 01/12/2015
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Summary

ApplicationELISA
Sample TestedSee PMID 22524708
SpeciesHuman

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 Verified Customer
01/12/2015
Application: ELISA
Species: Human
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Bioinformatics

Gene Symbol CCL2
Uniprot