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CCL2/JE/MCP-1 Antibody (123616R) [Unconjugated]

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Mouse splenocytes either unstimulated (open histogram) or stimulated with 1 ug/mL LPS overnight and 3 uM monensin for 2 hours (filled histogram) were stained with Recombinant Rat Anti-Mouse CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB479R), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Mouse splenocytes either unstimulated (open histogram) or stimulated with 1 ug/mL LPS overnight and 3 uM monensin for 2 hours (filled histogram) were stained with Recombinant Rat Anti-Mouse CCL2/JE/MCP-1 Monoclonal ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
123616R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4791U-100UG
Alexa Fluor 350
FAB479RU
Alexa Fluor 405
FAB4791V-100UG
Alexa Fluor 405
FAB479RV
Alexa Fluor 488
FAB4791G-100UG
Alexa Fluor 488
FAB479RG
Alexa Fluor 532
FAB479RX
Alexa Fluor 594
FAB479RT
Alexa Fluor 594
FAB4791T-100UG
Alexa Fluor 647
FAB4791R-100UG
Alexa Fluor 647
FAB479RR
Alexa Fluor 700
FAB4791N-100UG
Alexa Fluor 700
FAB479RN
Alexa Fluor 750
FAB4791S-100UG
Alexa Fluor 750
FAB479RS
Allophycocyanin
FAB479RA
Allophycocyanin/Cy7
FAB479RAPCCY7
Biotin
FAB479RB
DyLight 350
FAB479RD
DyLight 405
FAB479RE
DyLight 488
FAB479RK
DyLight 550
FAB479RL
DyLight 594
FAB479RM
DyLight 650
FAB479RW
DyLight 680
FAB479RY
DyLight 755
FAB479RZ
FITC
FAB479RF
HRP
FAB479RH
Janelia Fluor 549
FAB479RI
Janelia Fluor 646
FAB479RJ
PE
FAB479RP
PE/Atto594
FAB479RPEATT594
PE/Cy5.5
FAB479RPECY55
PE/Cy7
FAB479RPECY7
PerCP
FAB479RC
mFluor Violet 450 SE
FAB479RMFV450
mFluor Violet 500 SE
FAB479RMFV500
mFluor Violet 610 SE
FAB479RMFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB479R-SP
Carrier Free
MAB479R-500
Carrier Free
MAB479R-01M
Carrier Free
FAB4791V-100UG
Carrier Free
FAB4791U-100UG
Carrier Free
FAB4791T-100UG
Carrier Free
FAB4791S-100UG
Carrier Free
FAB4791R-100UG
Carrier Free
FAB4791N-100UG
Carrier Free
FAB4791G-100UG
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CCL2/JE/MCP-1 Antibody (123616R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant mouse CCL2
Gln24-Arg96
Accession # P10148
Specificity
Detects mouse CCL2 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Ccl2
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody (123616R) [Unconjugated]

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL2/JE/MCP-1 Antibody (123616R) [Unconjugated]
Species: Mu
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Bioinformatics

Gene Symbol Ccl2
Uniprot