Home » CCL14/HCC-1/HCC-3 » HCC-1 Antibodies » CCL14/HCC-1/HCC-3 Antibody (256409R) [Unconjugated]
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CCL14/HCC-1/HCC-3 Antibody (256409R) [Unconjugated]

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Recombinant Human CCL14a/HCC-1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCL14/HCC‑1/HCC‑3 Monoclonal Antibody (Catalog # MAB3241R). The ND50 is typically 0.5-2.5 µg/mL.
Recombinant Human CCL14a/HCC-1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
256409R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3241R-SP
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CCL14/HCC-1/HCC-3 Antibody (256409R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
Thr20-Asn109
Accession # NP_116738
Specificity
Detects human CCL14/HCC‑1/HCC‑3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from cell culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14/HCC-1/HCC-3 Antibody (256409R) [Unconjugated]

  • C-C motif chemokine 14
  • CCL14
  • chemokine (C-C motif) ligand 14
  • Chemokine CC-1/CC-3
  • chemokine CC-3
  • CKB1
  • FLJ16015
  • HCC-1
  • HCC-1(1-74)
  • HCC-1/HCC-3
  • HCC-3
  • hemofiltrate CC chemokine 1
  • MCIF
  • member 14
  • NCC2CC-1
  • NCC-2CKb1
  • new CC chemokine 2
  • SCYA14CC-3
  • Small-inducible cytokine A14

Background

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also known as HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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