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CCL14/HCC-1/HCC-3 Antibody (256409)

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 (1 Review)
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Recombinant Human CCL14a/HCC‑1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCL14/HCC‑1/HCC‑3 Monoclonal Antibody (Catalog # MAB3241). The ND50 is typically 0.5‑2.5 µg/mL.
Recombinant Human CCL14a/HCC‑1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
256409
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB3241-SP
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CCL14/HCC-1/HCC-3 Antibody (256409) Summary

Immunogen
E. coli-derived recombinant human CCL14/HCC‑1/HCC‑3
Thr20-Asn109
Accession # NP_116738
Specificity
Detects human CCL14/HCC‑1/HCC‑3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human CCL14a/HCC-1 Biotinylated Antibody (Catalog number BAF324)
Standard: Recombinant Human CCL14a/HCC-1 aa 20-93 (Catalog number 324-HC)
Reviewed Applications
Read 1 Review rated 4
using
MAB3241 in the following applications:
Publications
Read Publications using
MAB3241 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL14/HCC-1/HCC-3 Antibody (256409)

  • C-C motif chemokine 14
  • CCL14
  • chemokine (C-C motif) ligand 14
  • Chemokine CC-1/CC-3
  • chemokine CC-3
  • CKB1
  • FLJ16015
  • HCC-1
  • HCC-1(1-74)
  • HCC-1/HCC-3
  • HCC-3
  • hemofiltrate CC chemokine 1
  • MCIF
  • member 14
  • NCC2CC-1
  • NCC-2CKb1
  • new CC chemokine 2
  • SCYA14CC-3
  • Small-inducible cytokine A14

Background

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also known as HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL14/HCC-1/HCC-3 Antibody (MAB3241)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Neutralization, Western Blot.


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Neutralization
(1)
Western Blot
(1)
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(1)
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Review for CCL14/HCC-1/HCC-3 Antibody (MAB3241) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using MAB3241:
Filter by Applications
B/N
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Ruhul Choudhury
 B/N Rat 01/20/2017
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Summary

ApplicationBlock/Neutralize
Sample Testeduterine endothelial cells conditioned medium
SpeciesRat
LotKZD051511A

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Recent Reviews

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4
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2
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1
0
 
 Ruhul Choudhury
01/20/2017
Application: B/N
Species: Rat
read this review

Bioinformatics

Gene Symbol CCL14
Uniprot